Optimization for imaging through scattering media for confocal microscopes with divided elliptical apertures
| Autor: | Xinpei Zhu, Wei Gong, Hengjie Tang, Jiahao Wang, Ke Si, Yao Zheng, Chenxue Wu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Point spread function
Microscope Materials science Confocal General Physics and Astronomy 02 engineering and technology Lateral resolution Signal-To-Noise Ratio 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention 010309 optics Rats Sprague-Dawley Optics Confocal microscopy law 0103 physical sciences Animals Scattering Radiation General Materials Science Neurons Microscopy Confocal Scattering business.industry Detector General Engineering 1951 USAF resolution test chart General Chemistry 021001 nanoscience & nanotechnology Image Enhancement Rats 0210 nano-technology business |
| Zdroj: | Journal of biophotonics. 11(5) |
| ISSN: | 1864-0648 |
| Popis: | We develop a confocal system equipped with optimal elliptical apertures to improve axial point spread function and signal-to-background ratio (SBR) for different detector sizes. By adjusting the parameters of the elliptical apertures, the axial half width at half-maximum can be reduced to 4.986 (described in optical coordinates) and SBR can be improved to 0.176. We evaluate this system with the 1951 USAF resolution test chart and the primary cultured neuron from SD rat stained by Map-2, and observe better imaging performance, which indicates the potential applications in biological science. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text