Restricted antibody response toBordetella pertussisfilamentous hemagglutinin induced by whole-cell and acellular pertussis vaccines
| Autor: | Fazel Shokri, Jalal Khoshnoodi, Mahmood Jeddi-Tehrani, Sara Abdolmaleki, Saeed Zarei, Shadi Sadat Navabi, Hossein Asgarian-Omran, Hadi Alipour, Maryam Golara, Azam Hemmati |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Bordetella pertussis Filamentous haemagglutinin adhesin Enzyme-Linked Immunosorbent Assay Virulence factor law.invention Microbiology 03 medical and health sciences Vaccines Acellular Affinity chromatography Antigen law medicine Humans Virulence Factors Bordetella Adhesins Bacterial Child Pertussis Vaccine General Immunology and Microbiology biology Immunodominant Epitopes business.industry General Medicine biology.organism_classification Antibodies Bacterial Virology Healthy Volunteers 030104 developmental biology Infectious Diseases Vaccines Inactivated Child Preschool Recombinant DNA biology.protein Epitopes B-Lymphocyte Pertussis vaccine Female Antibody business medicine.drug |
| Zdroj: | Infectious Diseases. 48:127-132 |
| ISSN: | 2374-4243 2374-4235 1877-2250 |
| DOI: | 10.3109/23744235.2015.1093655 |
| Popis: | Filamentous hemagglutinin (FHA) is a principal virulence factor, an important immunogenic antigen of Bordetella pertussis, and a major component of many acellular pertussis vaccines. In the present study, the human antibody response to different regions of FHA was determined in healthy children and adults vaccinated with either whole-cell or acellular pertussis vaccines.To define the immunodominant regions of FHA, four overlapping recombinant fragments were expressed and produced in Escherichia coli and then purified by His-tagged based affinity chromatography. Two groups comprising healthy preschool children (n = 50) and adults (n = 26) were vaccinated with a single dose of commercial whole-cell and acellular DTaP vaccines, respectively. An antigen-based ELISA was applied to measure serum levels of anti-FHA antibody to both native and recombinant proteins in vaccinated volunteers.In both groups of vaccinated individuals, the anti-FHA antibody response was mainly directed against epitopes located within a fragment of FHA spanning amino acid residues 1877-2250 of the mature FHA molecule (p 0.001). No or little antibody was detected against the other recombinant segments of FHA.Our results suggest that the human antibody response to FHA is directed to an immunodominant region located within residues 1877-2250 of the FHA molecule. Characterization and epitope mapping of the major components of acellular pertussis vaccine and future modifications in vaccine formulation may improve its efficacy and protectivity. |
| Databáze: | OpenAIRE |
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