DUSP28 links regulation of Mucin 5B and Mucin 16 to migration and survival of AsPC-1 human pancreatic cancer cells
| Autor: | Jungsul Lee, Jae-Hoon Kim, Dae Gwin Jeong, Jeong Hun Yun |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Small interfering RNA Cell Survival Phosphatase Blotting Western Biology 03 medical and health sciences 0302 clinical medicine Cell Movement Pancreatic cancer Cell Line Tumor medicine Humans Phosphorylation MUC1 Oligonucleotide Array Sequence Analysis Mitogen-Activated Protein Kinase 1 Messenger RNA Mitogen-Activated Protein Kinase 3 Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Mucin General Medicine Transfection medicine.disease Molecular biology Mucin-5B Gene Expression Regulation Neoplastic Pancreatic Neoplasms 030104 developmental biology 030220 oncology & carcinogenesis CA-125 Antigen Focal Adhesion Kinase 1 Cancer research Dual-Specificity Phosphatases RNA Interference |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(9) |
| ISSN: | 1423-0380 |
| Popis: | The prognosis of pancreatic cancer has not improved despite considerable and continuous effort. Dual-specificity phosphatase 28 (DUSP28) is highly expressed in human pancreatic cancers and exerts critical effects. However, knowledge of its function in pancreatic cancers is extremely limited. Here, we demonstrate the peculiar role of DUSP28 in pancreatic cancers. Analysis using the Gene Expression Omnibus public microarray database indicated higher DUSP28, MUC1, MUC4, MUC5B, MUC16 and MUC20 messenger RNA (mRNA) levels in pancreatic cancers compared with normal pancreas tissues. DUSP28 expression in human pancreatic cancer correlated positively with those of MUC1, MUC4, MUC5B, MUC16 and MUC20. In contrast, there were no significant correlations between DUSP28 and mucins in normal pancreas tissues. Decreased DUSP28 expression resulted in down-regulation of MUC5B and MUC16 at both the mRNA and protein levels; furthermore, transfection with small interfering RNA (siRNA) for MUC5B and MUC16 inhibited the migration and survival of AsPC-1 cells. In addition, transfection of siRNA for MUC5B and MUC16 resulted in a significant decrease in phosphorylation of FAK and ERK1/2 compared with transfection with scrambled-siRNA. These results collectively indicate unique links between DUSP28 and MUC5B/MUC16 and their roles in pancreatic cancer; moreover, they strongly support a rationale for targeting DUSP28 to inhibit development of malignant pancreatic cancer. |
| Databáze: | OpenAIRE |
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