Anti-Inflammatory Effects of Conditioned Medium of Periodontal Ligament-Derived Stem Cells on Chondrocytes, Synoviocytes, and Meniscus Cells
| Autor: | Xue Yin, Yoh Sawatari, Franklin Garcia-Godoy, Oraya Vesvoranan, Chun Yuh Huang, Amanda Montoya, Valeria Londono |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.drug_class Periodontal Ligament Swine Interleukin-1beta Anti-Inflammatory Agents Arthritis Osteoarthritis Meniscus (anatomy) Biology Matrix (biology) Anti-inflammatory Culture Media Serum-Free 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine Chondrocytes Matrix Metalloproteinase 13 Conditioned medium medicine Periodontal fiber Animals Humans Meniscus Cells Cultured Tumor Necrosis Factor-alpha Stem Cells Cell Biology Hematology medicine.disease Synoviocytes MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Culture Media Conditioned Cancer research ADAMTS4 Protein 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem cells and development. 30(10) |
| ISSN: | 1557-8534 |
| Popis: | Osteoarthritis (OA) is the most common type of arthritis, afflicting millions of people in the world. Elevation of inflammatory mediators and enzymatic matrix destruction is often associated with OA. Therefore, the objective of this study was to investigate the effects of conditioned medium from periodontal ligament-derived stem cells (PDLSCs) on inflammatory and catabolic gene expressions of chondrocytes, synoviocytes, and meniscus cells under in vitro inflammatory condition. Stem cells were isolated from human periodontal ligaments. Conditioned medium was collected and concentrated 20 × . Chondrocytes, synoviocytes, and meniscus cells were isolated from pig knees and divided into four experimental groups: serum-free media, serum-free media+interleukin-1β (IL-1β) (10 ng/mL), conditioned media (CM), and CM+IL-1β. Protein content and extracellular vesicle (EV) miRNAs of CM were analyzed by liquid chromatography-tandem mass spectrometry and RNA sequencing, respectively. It was found that the IL-1β treatment upregulated the expression of IL-1β, tumor necrosis factor-α (TNF-α), MMP-13, and ADAMTS-4 genes in the three cell types, whereas PDLSC-conditioned medium prevented the upregulation of gene expression by IL-1β in all three cell types. This study also found that there was consistency in anti-inflammatory effects of PDLSC CM across donors and cell subcultures, while PDLSCs released several anti-inflammatory factors and EV miRNAs at high levels. OA has been suggested as an inflammatory disease in which all intrasynovial tissues are involved. PDLSC-conditioned medium is a cocktail of trophic factors and EV miRNAs that could mediate different inflammatory processes in various tissues in the joint. Introducing PDLSC-conditioned medium to osteoarthritic joints could be a potential treatment to prevent OA progression by inhibiting inflammation. |
| Databáze: | OpenAIRE |
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