Automated analysis of embryonic gene expression with cellular resolution in C. elegans
| Autor: | Zhirong Bao, Zhongying Zhao, Thomas J. Boyle, Barbara Mericle, Thomas J. Nicholas, Matthew J. Sandel, John I. Murray, Max E. Boeck, Robert H. Waterston |
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| Rok vydání: | 2008 |
| Předmět: |
Sensitivity and Specificity
Biochemistry Article Automation Genes Reporter Gene expression Animals Cell Lineage Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Gene Transcription factor Genetics Reporter gene biology Gene Expression Profiling Embryogenesis Gene Expression Regulation Developmental Reproducibility of Results Cell Biology biology.organism_classification Embryonic stem cell Cell biology Gene expression profiling Organ Specificity Biotechnology |
| Zdroj: | Nature Methods. 5:703-709 |
| ISSN: | 1548-7105 1548-7091 |
| Popis: | Automated imaging of the Caenorhabditis elegans embryo now allows monitoring of the timing and relative expression of individual reporter genes at single-cell resolution over almost all of embryonic development. Future systematic analysis could be used to reveal gene expression patterns of every cell during development. We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development. |
| Databáze: | OpenAIRE |
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