Determination of VX-G Analogue in Red Blood Cells via Gas Chromatography-Tandem Mass Spectrometry Following an Accidental Exposure to VX
| Autor: | Sandra A. Thomson, James T. Taylor, J.M. McGuire, C. E. Byers, Edward M. Jakubowski |
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| Rok vydání: | 2008 |
| Předmět: |
Erythrocytes
Potassium Compounds Health Toxicology and Mutagenesis Toxicology Mass spectrometry Tandem mass spectrometry Gas Chromatography-Mass Spectrometry Analytical Chemistry Fluorides Tandem Mass Spectrometry Humans Environmental Chemistry Chemical Warfare Agents Detection limit Chemical ionization Chemical Health and Safety Chromatography Gas Chromatography/Tandem Mass Spectrometry Chemistry Solid Phase Extraction Selected reaction monitoring Organothiophosphorus Compounds Environmental Exposure Environmental exposure Acetylcholinesterase Cholinesterase Inhibitors Gas chromatography–mass spectrometry Biomarkers Environmental Monitoring |
| Zdroj: | Journal of Analytical Toxicology. 32:73-77 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/32.1.73 |
| Popis: | A sensitive method for determining exposure to the chemical warfare agent VX is described in which the biomarker ethyl methylphosphonofluoridate (VX-G) is measured in red blood cells (RBCs) following treatment with fluoride ion using isotope-dilution gas chromatography-tandem mass spectrometry. The analyte was isolated via solid-phase extraction and detected using ammonia chemical ionization in the multiple reaction monitoring mode. A good linear relationship was obtained in the quantitative concentration range of 4 ng/mL to 1000 ng/mL with an absolute detection limit of < 1 pg on column. The method has been applied to the analysis of RBCs from a laboratory worker accidentally exposed to VX vapor. Detection and quantitation of VX-G were possible in samples taken as late as 27 days following exposure. |
| Databáze: | OpenAIRE |
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