Detection of the Trp-2027-Cys Mutation in Fluazifop-P-butyl–resistant Itchgrass (Rottboellia cochinchinensis) using High-Resolution Melting Analysis (HRMA)
| Autor: | Arturo Brenes-Angulo, Franklin Herrera-Murillo, Luis Gómez-Alpízar, Walter Barrantes-Santamaría, Rolbin Castillo-Matamoros |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Genetics Genotyping Grass weeds Rottboellia cochinchinensis Mutant Resistance Wild type Single-nucleotide polymorphism 04 agricultural and veterinary sciences Plant Science Biology ACCase inhibitors 01 natural sciences High Resolution Melt DNA sequencing 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Weed Agronomy and Crop Science 010606 plant biology & botany SNPs |
| Zdroj: | Weed Science, vol.66(3), pp.286-292 Kérwá Universidad de Costa Rica instacron:UCR |
| Popis: | Itchgrass [Rottboellia cochinchinensis (Lour.) Clayton] is recognized as one of the most noxious and troublesome annual weeds in tropical and subtropical regions. Acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides have been frequently used for managing R. cochinchinensis POST in a variety of crops, resulting in evolved resistance to these herbicides. Recently, resistance to fluazifop-P-butyl has been demonstrated for this weed, as the result of a G-to-C single-nucleotide polymorphism (SNP) that leads to the Trp-2027-Cys substitution in the ACCase enzyme. This study was conducted to develop a high-resolution melting analysis (HRMA) for the detection of the mutation underlying the Trp-2027-Cys substitution. The HRMA assay allowed differentiating between fluazifop-P-butyl–resistant (C mutant) and susceptible (G wild type) R. cochinchinensis plants. HRMA accuracy was confirmed with DNA sequencing of the target-site mutation, and no false positives or negatives were observed. Our results illustrated how HRMA is effective detecting the Trp-2027-Cys substitution in an R. cochinchinensis resistance, and how this technique can be of great value for developing high-throughput programs for monitoring evolution and dispersion of target site–based herbicide resistance at large scales. Universidad de Costa Rica/[]/UCR/Costa Rica UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro de Investigaciones Agronómicas (CIA) UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Estación Experimental Agrícola Fabio Baudrit Moreno (EEAFBM) |
| Databáze: | OpenAIRE |
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