Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12
| Autor: | Laura Francesca Pisani, C. Gorni, Paola Mariani, Paola Ramelli, Stefania Violini |
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| Rok vydání: | 2009 |
| Předmět: |
Homeobox protein NANOG
Clinical uses of mesenchymal stem cells Bone Marrow Cells Biology Stem cell marker Tendons medicine Animals Horses lcsh:QH573-671 Embryonic Stem Cells Stem cell transplantation for articular cartilage repair Homeodomain Proteins Extracellular Matrix Proteins lcsh:Cytology SOXB1 Transcription Factors Mesenchymal stem cell Membrane Proteins Cell Differentiation Mesenchymal Stem Cells Cell Biology Embryonic stem cell Cell biology medicine.anatomical_structure Gene Expression Regulation Bone Morphogenetic Proteins Proteoglycans Bone marrow Decorin Stem cell Octamer Transcription Factor-3 Biomarkers Research Article |
| Zdroj: | BMC Cell Biology BMC Cell Biology, Vol 10, Iss 1, p 29 (2009) |
| ISSN: | 1471-2121 |
| DOI: | 10.1186/1471-2121-10-29 |
| Popis: | Background Mesenchymal stem cells (MSCs) have been recently investigated for their potential use in regenerative medicine. MSCs, in particular, have great potential, as in various reports they have shown pluripotency for differentiating into many different cell types. However, the ability of MSCs to differentiate into tendon cells in vitro has not been fully investigated. Results In this study, we show that equine bone marrow mesenchymal stem cells (BM-MSCs), defined by their expression of markers such as Oct4, Sox-2 and Nanog, have the capability to differentiate in tenocytes. These differentiated cells express tendon-related markers including tenomodulin and decorin. Moreover we show that the same BM-MSCs can differentiate in osteocytes, as confirmed by alkaline phosphatase and von Kossa staining. Conclusion As MSCs represent an attractive tool for tendon tissue repair strategies, our data suggest that bone marrow should be considered the preferred MSC source for therapeutic approaches. |
| Databáze: | OpenAIRE |
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