Characterisation of Salmonella Enteritidis ST11 and ST1925 associated with human intestinal and extra-intestinal infections in Singapore

Autor: Kyaw Thu Aung, Wei Ching Khor, Kar Hui Ong, Wei Ling Tan, Zhi Ning Wong, Jia Quan Oh, Wai Kwan Wong, Brian Zi Yan Tan, Matthias Maiwald, Nancy Wen Sim Tee, Timothy Barkham, Tse Hsien Koh, Anders Dalsgaard, Swaine L. Chen, Joergen Schlundt, Lee Ching Ng
Přispěvatelé: School of Chemical and Biomedical Engineering, School of Biological Sciences, National Centre for Food Science, Singapore Food Agency, Environmental Health Institute, National Environment Agency, Nanyang Technological University Food Technology Centre
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Environmental Research and Public Health; Volume 19; Issue 9; Pages: 5671
Aung, K T, Khor, W C, Ong, K H, Tan, W L, Wong, Z N, Oh, J Q, Wong, W K, Tan, B Z Y, Maiwald, M, Tee, N W S, Barkham, T, Koh, T H, Dalsgaard, A, Chen, S L, Schlundt, J & Ng, L C 2022, ' Characterisation of Salmonella enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore ', International Journal of Environmental Research and Public Health, vol. 19, no. 9, 5671 . https://doi.org/10.3390/ijerph19095671
Popis: Salmonella Enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33-37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S.enteritidis. National Environmental Agency (NEA) Singapore Food Agency Published version This study was funded by the Singapore Food Agency, and the National Environment Agency, Singapore.
Databáze: OpenAIRE
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