Silver-ion reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in lipidic food analysis
| Autor: | Giacomo Dugo, Peter Q. Tranchida, Pavel Jandera, Paola Dugo, Vaclav Stanek, Luigi Mondello |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Chemical ionization
Chromatography Atmospheric pressure Chemistry Organic Chemistry Analytical chemistry Atmospheric-pressure chemical ionization General Medicine Reversed-phase chromatography Mass spectrometry Biochemistry High-performance liquid chromatography Lipids Mass Spectrometry Analytical Chemistry Two-dimensional chromatography Phase (matter) Chromatography High Pressure Liquid Food Analysis |
| Popis: | The triacylglycerol (TAG) profiles present in real world lipidic samples are usually quite complex and, as such, monodimensional high-performance liquid chromatographic (HPLC) techniques are inadequate when challenged with such matrices. In this respect, the complementary use of silver-ion (Ag) and non-aqueous (NA) reversed-phase (RP) HPLC can be exploited if thorough TAG separations are required. The present investigation reports the employment of a newly developed comprehensive LC (LC x LC) system, based on the different separation mechanisms of the aforementioned techniques, and applied to a rice oil sample. The approach was successful in the separation of a high number of solutes, otherwise unachievable through monodimensional LC. Furthermore, the use of atmospheric pressure chemical ionization mass spectrometry (APCI-MS), as detection system, provided a third analytical dimension boosting the identification power of the comprehensive chromatographic method. |
| Databáze: | OpenAIRE |
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