Microfluidic assay of the deformability of primitive erythroblasts
| Autor: | Sitong Zhou, Paul D. Kingsley, Yu-Shan Huang, Kathryn H. Cyr, Jiandi Wan, James Palis |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Classical Physics Biomedical Engineering Biology Cell membrane 03 medical and health sciences Colloid and Surface Chemistry Microfluidic channel hemic and lymphatic diseases medicine Nanotechnology General Materials Science Embryonic period Nanoscience & Nanotechnology Cytoskeleton Cellular biophysics Cell deformation Fluid Flow and Transfer Processes hemic and immune systems Hematology Condensed Matter Physics Cell biology 030104 developmental biology medicine.anatomical_structure Interdisciplinary Engineering circulatory and respiratory physiology Regular Articles |
| Zdroj: | Biomicrofluidics, vol 11, iss 5 |
| Popis: | Primitive erythroblasts (precursors of red blood cells) enter vascular circulation during the embryonic period and mature while circulating. As a result, primitive erythroblasts constantly experience significant hemodynamic shear stress. Shear-induced deformation of primitive erythroblasts however, is poorly studied. In this work, we examined the deformability of primitive erythroblasts at physiologically relevant flow conditions in microfluidic channels and identified the regulatory roles of the maturation stage of primitive erythroblasts and cytoskeletal protein 4.1 R in shear-induced cell deformation. The results showed that the maturation stage affected the deformability of primitive erythroblasts significantly and that primitive erythroblasts at later maturational stages exhibited a better deformability due to a matured cytoskeletal structure in the cell membrane. |
| Databáze: | OpenAIRE |
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