Analysis of cytomixis in tobacco microsporocytes with confocal laser scanning microscopy
| Autor: | Sergey Mursalimov, Yuri V. Sidorchuk, Elena V. Deineko |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nicotiana tabacum Plant Science Prophase 01 natural sciences Cytoplast Polyploidy 03 medical and health sciences Meiosis Cell Wall Tobacco Confocal laser scanning microscopy Nuclear migration Gametogenesis Plant Microscopy Confocal biology Cytomixis food and beverages Cell Biology General Medicine Plants Genetically Modified biology.organism_classification Cell biology 030104 developmental biology Micronucleus test Pollen 010606 plant biology & botany |
| Zdroj: | Protoplasma. 254:539-545 |
| ISSN: | 1615-6102 0033-183X |
| DOI: | 10.1007/s00709-016-0973-z |
| Popis: | Confocal laser scanning microscopy for the first time is used to examine the structure of the tobacco microsporocytes involved in the intercellular migration of nuclei (cytomixis). As is observed, the cytomictic channels are distributed over the surface of tobacco microsporocytes in a non-random manner and their number depends on the meiotic stage. Analysis of non-squash cells demonstrates the differences in cytological patterns of cytomixis in a normal meiosis of control tobacco plants (SR1 line) and the abnormal meiosis of polyploids. As a rule, two to three adjacent cells are involved in cytomixis during meiosis of control tobacco plants; after cytomixis, several micronuclei are formed in recipient cells; cytoplasts (enucleated cells) are rare; and polyads are undetectable. In the meiosis of polyploids, cytomixis is massive, with a larger number of cells (sometimes, over ten) involved in nuclear migration simultaneously; recipient cells on completion of cytomixis develop tens of micronuclei; cytoplasts and polyads are frequently detectable. |
| Databáze: | OpenAIRE |
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