Monitoring of engraftment and progression of acute lymphoblastic leukemia in individual NOD/SCID mice
| Autor: | Bart A Nijmeijer, Paul Mollevanger, Shama L van Zelderen-Bhola, Hanneke C Kluin-Nelemans, Roel Willemze, J.H.Frederik Falkenburg |
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| Přispěvatelé: | Human genetics, Dermatology |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Cancer Research Lymphoid Tissue Cell Transplantation Heterologous Spleen Nod Mice SCID Biology Translocation Genetic Mice In vivo Leukemic Infiltration Mice Inbred NOD Leukemia Myelogenous Chronic BCR-ABL Positive Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Genetics medicine Animals Chromosomes Human Humans Molecular Biology Cell growth Graft Survival Cell Biology Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Leukemia medicine.anatomical_structure Immunology Models Animal Disease Progression Female Bone marrow Blast Crisis Neoplasm Transplantation Homing (hematopoietic) |
| Zdroj: | Nijmeijer, B A, Mollevanger, P, Van Zelderen-Bhola, S L, Kluin-Nelemans, H C, Willemze, R & Falkenburg, J H F 2001, ' Monitoring of engraftment and progression of acute lymphoblastic leukemia in individual NOD/SCID mice ', Experimental Hematology, vol. 29, no. 3, pp. 322-329 . https://doi.org/10.1016/S0301-472X(00)00669-X Experimental Hematology, 29(3), 322-329. Elsevier Inc. |
| ISSN: | 0301-472X |
| DOI: | 10.1016/S0301-472X(00)00669-X |
| Popis: | Objective. The aim of this study was to develop an animal model for human acute lymphoblastic leukemia (ALL) in which the kinetics and characteristics of leukemia can be sequentially monitored in individual mice. Materials and Methods. NOD/SCID mice were inoculated intravenously with primary ALL. Progression of leukemia was monitored throughout the development of disease by determination of absolute leukemic cell counts (LCC) in peripheral blood. Results. LCC as low as 104 leukemic cells/mL blood could be detected. ALL cells from 5 of 5 patients engrafted, and after identification of the first leukemic cells in peripheral blood, LCC increased exponentially. Leukemic cells showed specificity of homing to spleen and bone marrow, and LCC strongly correlated with the level of leukemic engraftment in these organs throughout disease progression, demonstrating that LCC are representative for overall leukemic burden. Cytogenetic analysis of leukemic cells recovered after six successive in vivo transfers revealed no major karyotypic changes as compared to primary cells, and selection of the dominant clones was observed. This selection process was reflected by an increase in the rate of leukemic progression as compared to the first inoculation, demonstrating the accuracy with which kinetics of leukemic progression can be studied by determination of LCC. Conclusions. This model is suitable for detailed studies of kinetics and characteristics of ALL in vivo, and it may be useful for monitoring effects of novel therapeutic regimens. |
| Databáze: | OpenAIRE |
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