Identification and functional characterization of a solute carrier family 15, member 4 gene in Litopenaeus vannamei
| Autor: | Hai-Tao Yue, Ze-Zhi Zhang, Jianguo He, Feng-Hua Yuan, Yi-Hong Chen, Yonggui Chen, Kai Yuan, Shaoping Weng |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Gene Expression Regulation Viral Immunology Molecular Sequence Data Nerve Tissue Proteins Biology Cell Line 03 medical and health sciences 0302 clinical medicine Immune system White spot syndrome virus 1 Penaeidae Animals Humans Amino Acid Sequence Cloning Molecular RNA Small Interfering Gene Peptide sequence Reporter gene Innate immune system Sequence Homology Amino Acid NF-kappa B Membrane Transport Proteins Molecular biology DNA Virus Infections Immunity Innate Solute carrier family Open reading frame 030104 developmental biology 030220 oncology & carcinogenesis Vibrio Infections Unfolded protein response Unfolded Protein Response Vibrio parahaemolyticus Carrier Proteins Developmental Biology Antimicrobial Cationic Peptides |
| Zdroj: | Developmental and comparative immunology. 57 |
| ISSN: | 1879-0089 |
| Popis: | Innate immunity in shrimp is important in resisting bacterial infection. The NF-κB pathway is pivotal in such an immune response. This study cloned and functionally characterized the solute carrier family (SLC) 15 member A 4 (LvSLC15A4) gene in Litopenaeus vannamei. The open reading frame of LvSLC15A4 is 1, 902 bp long and encodes a putative 633-amino acid protein, which is localized in the plasma membrane and intracellular vesicular compartments. Results of the reporter gene assay showed that LvSLC15A4 upregulated NF-κB target genes, including the immediate-early gene 1 of white spot syndrome virus, as well as several antimicrobial peptide genes, such as pen4, CecA, AttA, and Mtk in S2 cells. Moreover, knocked-down expression of LvSLC15A4 reduced pen4 expression in L. vannamei. LvSLC15A4 down-regulation also increased the cumulative mortality of Vibrio parahemolyticus-infected L. vannamei. Furthermore, LvSLC15A4 expression was induced by unfolded protein response (UPR) in L. vannamei hematocytes. These results suggest that LvSLC15A4 participates in L. vannamei innate immunity via the NF-κB pathway and thus may be related to UPR. |
| Databáze: | OpenAIRE |
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