Stressor-induced ecdysis and thecate cyst formation in the armoured dinoflagellates Prorocentrum cordatum
| Autor: | Sofia A. Pechkovskaya, Sergei Skarlato, Ilya Pozdnyakov, Olga Matantseva, Mariia Berdieva, Vera Kalinina |
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| Rok vydání: | 2020 |
| Předmět: |
Water microbiology
0106 biological sciences 0301 basic medicine Ecophysiology Microorganism Population lcsh:Medicine Molting Microbiology 01 natural sciences Article 03 medical and health sciences Microscopy Electron Transmission Stress Physiological Centrifugation lcsh:Science Cellular microbiology education education.field_of_study Multidisciplinary biology Chemistry 010604 marine biology & hydrobiology lcsh:R Dinoflagellate biology.organism_classification Cell biology 030104 developmental biology Theca Ecdysis Dinoflagellida Ultrastructure Cyst formation lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-17 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-75194-3 |
| Popis: | Ecdysis, the process of extensive cell covering rearrangement, represents a remarkable physiological trait of dinoflagellates. It is involved in the regulation of the population and bloom dynamics of these microorganisms, since it is required for the formation of their thin-walled cysts. This study presents laboratory data on ecdysis in Prorocentrum cordatum, a harmful dinoflagellate species of high environmental significance. We studied external stressors triggering this process and changes in the cell ultrastructure accompanying it. Our experiments showed that mass ecdysis and formation of cysts in P. cordatum could be induced by centrifugation, temperature decrease, changes in salinity, and treatment by 2,6-dichlorobenzonitrile, whereas temperature increase, changes in pH and treatment by tetracycline did not have this effect. Obtained cysts of P. cordatum did not contain the pellicular layer and were formed in the end of the first stage of this process, i.e. removal of the plasma membrane and the outer amphiesmal vesicle membrane, whereas its second stage, removal of theca, represented excystment. Based on our findings, we conclude that such cysts can be attributed to thecate cysts and suggest P. cordatum as a promising model organism for the investigation of cellular and molecular aspects of ecdysis in dinoflagellates. |
| Databáze: | OpenAIRE |
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