In situ formation and scanning electrochemical microscopy assisted positioning of NO-sensors above human umbilical vein endothelial cells for the detection of nitric oxide release
| Autor: | Joshua Oni, Mathieu Etienne, Sonnur Isik, Wolfgang Schuhmann, Fethi Bedioui, Sabine Reiter, Alain Pailleret |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
education.field_of_study
Population Nanotechnology Electrocatalyst Amperometry Umbilical vein Nitric oxide lcsh:Chemistry Microelectrode Scanning electrochemical microscopy chemistry.chemical_compound chemistry lcsh:Industrial electrochemistry lcsh:QD1-999 Electrochemistry Biophysics education Biosensor lcsh:TP250-261 |
| Zdroj: | Electrochemistry Communications, Vol 5, Iss 10, Pp 847-852 (2003) |
| ISSN: | 1388-2481 |
| Popis: | Pt microelectrodes (50 μm diameter) were positioned by means of scanning electrochemical microscopy assisted z-approach curves and in situ modified with nickel tetrasulfonated phthalocyanine tetrasodium salt as electrocatalytic layer for the specific oxidative detection of nitric oxide. The thus modified electrodes were then moved over a layer of adherently growing human umbilical vein endothelial cells (HUVEC) in order to amperometrically detect nitric oxide (NO) released from the cells upon stimulation with bradykinin. This approach actually takes advantage of the use of SECM to define a sequential procedure that enables the in situ functionalisation of the SECM tip thus allowing to accurately control the separation between the functionalised SECM tip and the cell population. Keywords: Nitric oxide, SECM, Modified electrodes, HUVEC, NO sensor |
| Databáze: | OpenAIRE |
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