Nanocomposite sponges for enhancing intestinal residence time following oral administration

Autor: Veronica La Padula, Ana Grenha, Giovanna Lollo, Filipa Guerreiro, Yves Chevalier, Laurent David, Alexandra Montembault, Adem Gharsallaoui, Annalisa Rosso, Valentina Andretto, Jacqueline Sidi-Boumedine, Stéphanie Briançon, David Kryza
Přispěvatelé: Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Université de Lyon, Hospices Civils de Lyon (HCL), Ingénierie des Matériaux Polymères (IMP), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Intestinal drug delivery
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Kinetics
Pharmaceutical Science
Nanoparticle
Administration
Oral
02 engineering and technology
Nanocomposites
Chitosan
03 medical and health sciences
chemistry.chemical_compound
Mice
Drug Delivery Systems
Nanoemulsion
Oral administration
Microscopy
Animals
Humans
Tissue Distribution
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Chitosan sponge
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
Nanocomposite
biology
Chemistry
Mucin
021001 nanoscience & nanotechnology
biology.organism_classification
Intestines
Sponge
[SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology
Biophysics
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Nanoparticles
Caco-2 Cells
0210 nano-technology
Zdroj: Journal of Controlled Release
Journal of Controlled Release, Elsevier, 2021, ⟨10.1016/j.jconrel.2021.04.004⟩
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 0168-3659
Popis: In this work, nanocomposites that combine mucopenetrating and mucoadhesive properties in a single system are proposed as innovative strategy to increase drug residence time in the intestine following oral administration. To this aim, novel mucoadhesive chitosan (CH) sponges loaded with mucopenetrating nanoemulsions (NE) were developed via freeze-casting technique. The NE mucopenetration ability was determined studying the surface affinity and thermodynamic binding of the nanosystem with mucins. The ability of nanoparticles to penetrate across a preformed mucins layer was validated by 3D-time laps Confocal Laser Scanning Microscopy imaging. Microscopy observations (Scanning Electron Microscopy and Optical Microscopy) showed that NE participated in the structure of the sponge affecting its stability and in vitro release kinetics. When incubated with HCT 116 and Caco-2 cell lines, the NE proved to be cytocompatible over a wide concentration range. Finally, the in vivo biodistribution of the nanocomposite was evaluated after oral gavage in healthy mice. The intestinal retention of NE was highly enhanced when loaded in the sponge compared to the NE suspension. Overall, our results demonstrated that the developed nanocomposite sponge is a promising system for sustained drug intestinal delivery. Portuguese Foundati on for Science and Technology and European Commission: 42306YB; Portuguese Foundation for Science and Technology: UID/Multi/04326/2020 info:eu-repo/semantics/publishedVersion
Databáze: OpenAIRE
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