Gross, Histologic, Cytochemical, and Immunocytochemical Study of Medullary Thyroid Carcinoma in Sixteen Dogs
| Autor: | P. H. Lieberman, A. K. Patnaik |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Calcitonin
Male 0301 basic medicine Pathology medicine.medical_specialty 040301 veterinary sciences medicine.medical_treatment Synaptophysin Vimentin Biology 0403 veterinary science Thyroid carcinoma 03 medical and health sciences Dogs medicine Animals Dog Diseases Thyroid Neoplasms General Veterinary Histocytochemistry Carcinoma Membrane Proteins 04 agricultural and veterinary sciences Immunohistochemistry 030104 developmental biology Giant cell Phosphopyruvate Hydratase biology.protein Oxyphil cell (pathology) Female Thyroglobulin Clear cell |
| Zdroj: | Veterinary Pathology. 28:223-233 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/030098589102800306 |
| Popis: | The gross, histomorphologic, cytochemical, and immunocytochemical findings in 16 dogs with medullary thyroid carcinoma were evaluated. Grossly, the neoplasms were encapsulated, firm, lobulated, and grey-white to tan. The typical histologic pattern was groups or sheets of round to polygonal cells with fibrovascular stroma, which was thickened and hyalinized in places. Variants of clear cell (two dogs), giant cell (one dog), and oxyphil cell (one dog) types were also seen. In all 16 dogs, Grimelius-stained sections of the neoplasms revealed intracytoplasmic silver granules; ten tumors contained amyloid and four contained mucin. Immunohistochemically, the neoplasms reacted to AE1/AE3 ( n = 13), S-100 protein ( n = 5), neuron specific enolase ( n = 14), synaptophysin ( n = 11), calcitonin ( n = 16), somatostatin ( n = 4), gastrin ( n = 7), and serotonin ( n = 6). Only one neoplasm was positive for vimentin. None of the neoplasms reacted to antibodies for neurofilaments, thyroglobulin, insulin, glucagon, or adrenocorticotrophic hormone. Eleven neoplasms contained multiple (two to four) peptides, in various combinations. It was concluded that in dogs, gross and histologic features can be used to distinguish medullary thyroid carcinoma from other thyroid malignancies. Cytochemical and immunocytochemical studies with neuron specific enolase, synaptophysin, and calcitonin can be used to establish the diagnosis of medullary thyroid carcinoma in dogs. |
| Databáze: | OpenAIRE |
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