Separation of nadolol racemates by high pH reversed-phase preparative chromatography
| Autor: | Alírio E. Rodrigues, Luís S. Pais, António E. Ribeiro, Rami S. Arafah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Diethylamine
Chromatography Filtration and Separation 02 engineering and technology 021001 nanoscience & nanotechnology Analytical Chemistry Solvent chemistry.chemical_compound Nadolol Adsorption 020401 chemical engineering chemistry Phase (matter) medicine Reversed-phase preparative chromatography Multicomponent separation 0204 chemical engineering Simulated moving bed Enantiomer 0210 nano-technology medicine.drug |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | The separation of nadolol racemates under high pH reversed-phase preparative chromatography is presented for the first time. Three Waters C18 adsorbents (XBridge, Shield and XSelect) are compared for the separation of nadolol racemates using ethanol:water:diethylamine solvent mixtures. Experimental and simulation results are presented to compare the separation performances at preparative scale using both the fixed-bed and the simulated moving bed operations. The Waters XBridge C18 adsorbent and an ethanol:water:diethylamine solvent mixture are selected as a good option for the separation of nadolol racemates. The validated methodology allows the separation of a multicomponent nadolol feed mixture composed by four stereoisomers into two pure racemates (two pairs of enantiomers). This work introduces the potential of using an initial achiral separation step in the global strategy for the complete multicomponent separation of the four nadolol stereoisomers. This work is a result of: Project “AIProcMat@N2020 - Advanced Industrial Processes and Materials for a Sustainable Northern Region of Portugal 2020”, with the reference NORTE-01-0145-FEDER-000006, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); Associate Laboratory LSRE-LCM - UID/EQU/50020/2019 - funded by national funds through FCT/MCTES (PIDDAC). Rami S. Arafah is supported by a PhD Grant of Fundação para a Ciência e a Tecnologia (SFRH/BD/137966/2018). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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