Vapourized hydrogen peroxide decontamination in a hospital setting inactivates SARS-CoV-2 and HCoV-229E without compromising filtration efficiency of unexpired N95 respirators
| Autor: | Stephenie Naugler, Shannon E. McCaw, Furkan Guvenc, Ayoob Ghalami, Steven Hayes, Rachel Tyli, Betty P.K. Poon, Natasha Christie-Holmes, Rita A. Kandel, Amit Weiner, Mary Speck, Ori D. Rotstein, Scott D. Gray-Owen, James A. Scott, Allen Rainville, Patrick Budylowski, Samira Mubareka |
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| Rok vydání: | 2021 |
| Předmět: |
business.product_category
N95 Respirators Epidemiology viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) law.invention Bioburden chemistry.chemical_compound Coronavirus 229E Human law Equipment Reuse Major Article Humans Medicine Respirator Hydrogen peroxide Pandemics Decontamination Filtration SARS-CoV-2 business.industry N95 Health Policy Public Health Environmental and Occupational Health COVID-19 virus diseases HCoV-229E Hydrogen Peroxide Human decontamination Sterilization (microbiology) Pulp and paper industry Hospitals Disinfection hydrogen peroxide vapor respiratory protection Infectious Diseases chemistry Vaporized hydrogen peroxide mask reprocessing business |
| Zdroj: | American Journal of Infection Control |
| ISSN: | 0196-6553 |
| DOI: | 10.1016/j.ajic.2021.07.012 |
| Popis: | Background The COVID-19 pandemic highlighted the need for evidence-based approaches to decontamination and reuse of N95 filtering facepiece respirators (FFRs). We sought to determine whether vapourized hydrogen peroxide (VHP) reduced SARS-CoV-2 bioburden on FFRs without compromising filtration efficiency. We also investigated coronavirus HCoV-229E as a surrogate for decontamination validation testing. Methods N95 FFRs were laced with SARS-CoV-2 or HCoV-229E and treated with VHP in a hospital reprocessing facility. After sterilization, viral burden was determined using viral outgrowth in a titration assay, and filtration efficiency of FFRs was tested against ATSM F2299 and NIOSH TEB-STP-APR-0059. Results Viable SARS-CoV-2 virus was not detected after VHP treatment. One replicate of the HCoV-229E laced FFRs yielded virus after processing. Unexpired N95 FFRs retained full filtration efficiency after VHP processing. Expired FFRs failed to meet design-specified filtration efficiency and therefore are unsuitable for reprocessing. Discussion In-hospital VHP is an effective decontaminant for SARS-CoV-2 on FFRs. Further, filtration efficiency of unexpired respirators is not affected by this decontamination process. Conclusions VHP is effective in inactivating SARS-CoV-2 on FFRs without compromising filtration efficiency. HCoV-229E is a suitable surrogate for SARS-CoV-2 for disinfection studies. |
| Databáze: | OpenAIRE |
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