Protective effect of EGTA on rat gastric membranes enriched in (H+-K+)-ATPase
| Autor: | Wha Bin Im, David P. Blakeman |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Membrane permeability
ATPase Biophysics In Vitro Techniques Phospholipase Biochemistry Permeability H(+)-K(+)-Exchanging ATPase Valinomycin chemistry.chemical_compound Microsomes Gastric mucosa medicine Animals Egtazic Acid Molecular Biology Adenosine Triphosphatases HEPES biology Chemistry Intracellular Membranes Cell Biology Hydrogen-Ion Concentration Rats EGTA medicine.anatomical_structure Membrane Gastric Mucosa Phospholipases biology.protein Calcium Ethylene Glycols |
| Zdroj: | Biochemical and Biophysical Research Communications. 108:635-639 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(82)90876-2 |
| Popis: | Rat gastric membranes enriched in (H+-K+)-ATPase, when prepared in the presence of 1 m M ethyleneglycol-bis-(β-aminoethyl ether)N,N′-tetraacetic acid, showed the ability to accumulate H+ ions upon addition of ATP, KCl, and valinomycin. The membranes were largely impermeable to K+ and Cl−. In contrast, the rat membranes prepared without the Ca2+ chelator lost the ability to develop a pH gradient because of the membrane leakiness to H+. A majority of these membrane vesicles became also permeable to K+. We suggest that the calcium chelator preserved the gastric membrane permeability barrier during isolation by inhibiting various Ca2+-dependent phospholipases in rat gastric mucosa. |
| Databáze: | OpenAIRE |
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