Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens
| Autor: | Daiki Kudo, Yoshihiro Takizumi, Hiroyuki Ishimaru, Yuling Bai, Koji Kakutani, Yoshihiro Takikawa, Teruo Nonomura, Tomoko Suzuki, Yoshinori Matsuda, Tomoe Murakami |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hyphal growth Hypha Erysiphe trifoliorum Plant Science Horticulture 01 natural sciences Conidium Hypersensitive cell death 03 medical and health sciences Podosphaera xanthii Laboratorium voor Plantenveredeling Botany Wild tomato Appressorium Delayed cell death biology fungi food and beverages biology.organism_classification Trichome Pseudoidium neolycopersici Plant Breeding 030104 developmental biology Papilla formation EPS Solanum Agronomy and Crop Science Powdery mildew 010606 plant biology & botany |
| Zdroj: | European Journal of Plant Pathology 150 (2018) 1 European Journal of Plant Pathology, 150(1), 115-125 |
| ISSN: | 0929-1873 |
| Popis: | The present study aimed to explore the possibility of using the type I trichomes of tomato (Solanum lycopersicum) to monitor the infection processes of powdery mildews by microscopy. Individual trichome cells of two tomato genotypes were inoculated with pathogenic and non-pathogenic powdery mildew species, Pseudoidium neolycopersici, Erysiphe trifoliorum and Podosphaera xanthii. On the trichome cells of the tomato cultivar Moneymaker, hyphae of the pathogenic Pseudoidium neolycopersici (isolates KTP-03 and KTP-04) grew vigorously; whereas hyphal growth of the non-pathogenic Erysiphe trifoliorum and Podosphaera xanthii ceased after appressorium formation, which was associated with papilla formation and hypersensitive cell death, respectively. Similar infection processes of the tested powdery mildews were seen in Moneymaker epidermal cells. Therefore, tomato trichomes are suitable for analysing, at individual cell level, the infection processes of different pathotypes of powdery mildews and for observing the cytological responses of plants by non-pathogenic powdery mildews. On the other hand, it was observed that both isolates KTP-03 and KTP-04 failed to produce conidiophores on the hyphae elongating on Moneymaker trichomes. Similarly, no conidiophores were produced on the hyphae elongating on trichomes of Solanum peruvianum LA2172, which is resistant to KTP-03 and susceptible to KTP-04. Interestingly, delayed cell death occurred in LA2172 epidermal cells, which were attacked by KTP-03 hyphae elongating from trichomes and conidiophores were formed on new hyphae growing from the leaf epidermal cells. Thus, leaf trichomes and epidermal cells of the wild tomato species LA2172 reacted differently to the avirulent isolate KTP-03. |
| Databáze: | OpenAIRE |
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