Fatty Acid Profiles for Differentiating Growth Medium Formulations Used to Culture Bacillus cereus T-strain Spores
| Autor: | James M. Robertson, Jason D. Bannan, Christopher J. Ehrhardt, Devonie L. Murphy |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Spores Bacterial Growth medium biology fungi Fatty Acids Bacillus cereus Fatty acid Discriminant Analysis biology.organism_classification Pathology and Forensic Medicine Spore Culture Media chemistry.chemical_compound chemistry Biochemistry Tryptone Casein Peptones Genetics Soybean Proteins Soy protein Fatty acid methyl ester Biomarkers |
| Zdroj: | Journal of forensic sciences. 60(4) |
| ISSN: | 1556-4029 |
| Popis: | Microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. In this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. Bacillus cereus T-strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain-heart infusion. Cellular biomass was profiled with fatty acid methyl ester (FAME) analysis. Results showed peptone cultures produced spores enriched in straight-chained lipids. Tryptone cultures produced spores enriched in branched-odd lipids when compared with peptone, soy, and brain-heart formulations. The observed FAME variation was used to construct a set of discriminant functions that could help identify the nutrients in a culturing recipe for an unknown spore sample. Blinded classification tests were most successful for spores grown on media containing peptone and tryptone, showing 88% and 100% correct identification, respectively. |
| Databáze: | OpenAIRE |
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