Protective effect of polyphenols in an inflammatory process associated with experimental pulmonary fibrosis in mice
| Autor: | Marika Cordaro, Giuseppe Bruschetta, Jose Maria Zubelia, Rosalia Crupi, Daniela Impellizzeri, Rosalba Siracusa, Emanuela Esposito, Virginia Motilva, Salvatore Cuzzocrea, Antonio Montero Alcaide, Elena Talero |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pulmonary Fibrosis Xanthones Medicine (miscellaneous) Apoptosis Respiratory Mucosa Lung injury Pharmacology Resveratrol medicine.disease_cause Antioxidants Random Allocation chemistry.chemical_compound Stilbenes Pulmonary fibrosis medicine Animals Vitis Mangiferin Lung Mice Inbred ICR Mangifera Nutrition and Dietetics medicine.diagnostic_test Plant Extracts Nitrotyrosine Anti-Inflammatory Agents Non-Steroidal Polyphenols food and beverages medicine.disease Apoptosis BAL bronchoalveolar lavage BLM bleomycin COX-2 cyclo-oxygenase-2 DHQ dihydroquercetin IPF idiopathic pulmonary fibrosis Inflammation Lung injury MPO myeloperoxidase Oxidative stress PARP poly (ADP-ribose) polymerase Polyphenols ROS reactive oxygen species iNOS inducible NO synthase pERK phosphorylated extracellular signal-regulated kinase Animals Anti-Inflammatory Agents Non-Steroidal Antioxidants Apoptosis Lung Male Mangifera Mice Inbred ICR Neutrophil Infiltration Plant Extracts Plant Leaves Polyphenols Pulmonary Fibrosis Quercetin Random Allocation Respiratory Mucosa Stilbenes Vitis Xanthones Dietary Supplements Disease Models Animal Plant Leaves Disease Models Animal Bronchoalveolar lavage Neutrophil Infiltration chemistry Dietary Supplements Immunology Quercetin Oxidative stress |
| Zdroj: | British Journal of Nutrition. 114:853-865 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s0007114515002597 |
| Popis: | Polyphenols have been described to have a wide range of biological activities, and many reports, published during recent years, have highlighted the beneficial effects of phenolic compounds, illustrating their promising role as therapeutic tools in several acute and chronic disorders. The purpose of study was to evaluate, in an already-assessed model of lung injury caused by bleomycin (BLM) administration, the role of resveratrol and quercetin, as well as to explore the potential beneficial properties of a mango leaf extract, rich in mangiferin, and a grape leaf extract, rich in dihydroquercetin (DHQ), on the same model. Mice were subjected to intra-tracheal administration of BLM, and polyphenols were administered by oral route immediately after BLM instillation and daily for 7 d. Treatment with resveratrol, mangiferin, quercetin and DHQ inhibited oedema formation and body weight loss, as well as ameliorated polymorphonuclear infiltration into the lung tissue and reduced the number of inflammatory cells in bronchoalveolar lavage fluid. Moreover, polyphenols suppressed inducible nitric oxide synthase expression, and prevented oxidative and nitroxidative lung injury, as shown by the reduced nitrotyrosine and poly (ADP-ribose) polymerase levels. The degree of apoptosis, as evaluated by Bid and Bcl-2 balance, was also suppressed after polyphenol treatment. Finally, these natural products down-regulated cyclo-oxygenase-2, extracellular signal-regulated kinase phosphorylated expression and reduced NF-κBp65 translocation. Our findings confirmed the anti-inflammatory effects of resveratrol and quercetin in BLM-induced lung damage, and highlight, for the first time, the protective properties of exogenous administration of mangiferin and DHQ on experimental pulmonary fibrosis. |
| Databáze: | OpenAIRE |
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