Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
Autor: | Zongchao Jia, Prem Khanal, Xiaolong Yang |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
MST1 Cellular differentiation Science Apoptosis Protein Serine-Threonine Kinases Article Serine 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Neoplasms Humans Hippo Signaling Pathway Cysteine Protein kinase A Tissue homeostasis Adaptor Proteins Signal Transducing Cell Proliferation Hippo signaling pathway Multidisciplinary Chemistry Intracellular Signaling Peptides and Proteins Cell Differentiation YAP-Signaling Proteins Phosphoproteins Hedgehog signaling pathway 3. Good health Cell biology 030104 developmental biology 030220 oncology & carcinogenesis Transcriptional Coactivator with PDZ-Binding Motif Proteins Trans-Activators Medicine Protein Multimerization Signal Transduction Transcription Factors |
Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Hippo signalling pathway is an emerging signalling pathway that plays important roles in organ size control, tumorigenesis, metastasis, stress response, apoptosis, stem cell differentiation and renewal during development and tissue homeostasis. Recent studies reported that human serine/threonine protein kinase, Mst1, a core component of the Hippo pathway can be activated through formation of homodimer. However, it is still unclear whether or not other components of the Hippo pathway are also regulated through dimerization. Here we provide the first evidence that Hippo components and oncoprotein YAP2L and TAZ can form homodimer in vitro and in vivo by forming disulphide bond through cysteine residue(s). We have also shown that the homodimers of YAP2L/TAZ are more stable and showed more oncogenic behaviour than their corresponding monomers as revealed by colony formation and cell transformation assay. Since cysteine post-translational regulation plays important roles in redox signalling, tumorigenesis and drug resistance, further studies on the functional effect of this dimerization through post-translational modulation of cysteine residues in YAP2L/TAZ will provide a significant contribution to our understanding of the roles of YAP2L/TAZ in cancer development and therapy. |
Databáze: | OpenAIRE |
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