Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17)
| Autor: | Rima Z. Shaikhutdinova, Svetlana V. Dentovskaya, Aleksandr G. Bogun, Sergei A. Ivanov, Yuriy A. Knirel, Olga V. Sizova, Angelina A. Kislichkina, Alexander S. Shashkov, Lidia A. Kadnikova, Anna N. Kondakova |
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| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Stereochemistry 030106 microbiology Polysaccharide medicine.disease_cause Biochemistry Fucose Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Gene cluster medicine Yersinia intermedia Escherichia coli chemistry.chemical_classification biology Chemistry Organic Chemistry O Antigens General Medicine biology.organism_classification Yersinia Sephadex Multigene Family Two-dimensional nuclear magnetic resonance spectroscopy Bacteria |
| Zdroj: | Carbohydrate Research. 460:51-56 |
| ISSN: | 0008-6215 |
| Popis: | Lipopolysaccharide was isolated from bacteria Yersinia intermedia H9-36/83 (O:17) and degraded with mild acid to give an O-specific polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar analysis and 1D and 2D NMR spectroscopy. The polysaccharide was found to contain 3-deoxy-3-[( R )-3-hydroxybutanoylamino]- d -fucose ( d -Fuc3N R 3Hb) and the following structure of the heptasaccharide repeating unit was established: Download high-res image (83KB) Download full-size image The structure established is consistent with the gene content of the O-antigen gene cluster. The O-polysaccharide structure and gene cluster of Y. intermedia are related to those of Hafnia alvei 1211 and Escherichia coli O:103. |
| Databáze: | OpenAIRE |
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