Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury
| Autor: | Wai-Hung Sit, Jennifer Man-Fan Wan, Kelvin Hoi-Man Fu, Cheuk-Lun Lee, Daniel Kwong-On Chan |
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| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide medicine.medical_treatment T-Lymphocytes Immunology Anti-Inflammatory Agents CD4-CD8 Ratio Transferases (Other Substituted Phosphate Groups) Salvia miltiorrhiza Cell Line Sepsis chemistry.chemical_compound Mice Immunophenotyping medicine Immunology and Allergy Animals Humans RNA Messenger Pharmacology Liver injury Mice Inbred BALB C business.industry Tumor Necrosis Factor-alpha Macrophages medicine.disease Cytokine chemistry Gene Expression Regulation Liver Toxicity Tumor necrosis factor alpha Plant Preparations business Spleen Drugs Chinese Herbal |
| Zdroj: | International immunopharmacology. 6(5) |
| ISSN: | 1567-5769 |
| Popis: | Lipopolysaccharide (LPS) has been implicated as one of the major cause of Gram-negative bacteria-induced sepsis that are life-threatening syndromes occurring in intensive care unit patients. Many natural products derived from medicinal plants may contain therapeutic values on protecting endotoxemia-induced sepsis by virtue their ability to modulate multiple pro-inflammatory cytokines. In the present study, we show that Salvia miltiorrhiza (SM) BUNGE or Danshen, used in treatment of various systemic and surgical infections in the hospitals of China, was able to block the lethal toxicity of LPS in mice via suppression of TNF-alpha release and protection on liver injury. The ability of SM to suppress LPS-induced TNF-alpha release is further confirmed by in vitro experiments conducted on human peripheral blood leukocytes (PBL) and the RAW 264.7 macrophage cell line. Immunophenotyping by flow cytometry shows improved T-helper cell (CD4) and T-suppressor cells (CD8) ratio in SM-treated PBL and splenocytes of LPS-challenged mice. The drop in plasma glutamate-pyruvate transaminase (GPT) induced by LPS provides evidence that SM can protect hepatic damage. The present study explains some known biological activities of SM, and supports the clinical application of SM in the prevention of inflammatory diseases induced by Gram-negative bacteria. |
| Databáze: | OpenAIRE |
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