Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum
| Autor: | Mutsumi Takagi, Itaru Shioya, Yu Aizu, Masashi Fujiwara |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Serum
medicine.medical_specialty Cell Culture Techniques Bioengineering CHO Cells Applied Microbiology and Biotechnology Biology Suspension culture Culture Media Serum-Free law.invention Andrology Cricetulus Serum free law Cricetinae Internal medicine medicine Animals Fetus Cell growth Chinese hamster ovary cell Fishes Endocrinology Recombinant DNA Fish Cattle Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 109:307-309 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2009.08.481 |
| Popis: | The effects of heat treatment and concentration of fish serum (FS) on cell growth in a suspension culture of recombinant Chinese hamster ovary (CHO) 1-15 500 (ATCC CRL-9606) cells were investigated. An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect