Optimization of the32P-Postlabeling/Thin Layer Chromatography Assay (32P-TLC) for In Vitro Detection of 8-Oxo-Deoxyguanosine as a Biomarker of Oxidative DNA Damage

Autor:	Imed Maatouk, Noureddine Bouaïcha, François Périn, Marie-José Plessis
Rok vydání:	2006
Předmět:	Chromatography Chemistry Health Toxicology and Mutagenesis DNA oxidation Toxicology medicine.disease_cause Molecular biology Thin-layer chromatography In vitro Oxidative dna damage Biomarker chemistry.chemical_compound medicine Deoxyguanosine heterocyclic compounds Oxidative stress DNA
Zdroj:	Toxicology Mechanisms and Methods. 16:313-322
ISSN:	1537-6524 1537-6516
Popis:	During the last years, much attention was focused on the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a marker of oxidative DNA damage. Among various analytical techniques, the (32)P-postlabeling assay has been applied in determination of 8-oxo-dG. However, artefactual DNA oxidation could take place during the work-up procedures leading to over-estimate the level of 8-oxo-dG. In the present study, we optimized the (32)P-postlabelling assay with thin layer chromatography to measure 8-oxo-dG in standard samples of 8-oxo-dG, calf thymus DNA and primary cultured rat hepatocytes. The background levels of 8-oxo-dG in calf thymus DNA and in primary cultured rat hepatocytes were lesser than those determined by the previously described (32)P-postlabeling procedures and were in the range of those determined by chromatography methods (GC-MS, HPLC-MS).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b19856b2be93b69a8aff0885416a573d https://doi.org/10.1080/15376520600616909 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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