The Drosophila HP1 Homolog Rhino Is Required for Transposon Silencing and piRNA Production by Dual-Strand Clusters
| Autor: | Nadine Schultz, Birgit S. Koppetsch, Jaspreet S. Khurana, Hualin Xi, Fan Zhang, Hervé Seitz, Carla Andrea Klattenhoff, Anetta Nowosielska, Zhiping Weng, Phillip D. Zamore, Chengjian Li, William E. Theurkauf, Jia Xu, Soohyun Lee |
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| Rok vydání: | 2009 |
| Předmět: |
Transposable element
endocrine system Chromatin Immunoprecipitation Transcription Genetic Heterochromatin Chromosomal Proteins Non-Histone Piwi-interacting RNA General Biochemistry Genetics and Molecular Biology Article RasiRNA Animals Drosophila Proteins Gene Silencing RNA Small Interfering Genetics biology urogenital system Biochemistry Genetics and Molecular Biology(all) RNA biology.organism_classification STEMCELL Drosophila melanogaster DNA Transposable Elements Heterochromatin protein 1 Drosophila Protein |
| Zdroj: | Cell. 138(6):1137-1149 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2009.07.014 |
| Popis: | SummaryPiwi-interacting RNAs (piRNAs) silence transposons and maintain genome integrity during germline development. In Drosophila, transposon-rich heterochromatic clusters encode piRNAs either on both genomic strands (dual-strand clusters) or predominantly one genomic strand (uni-strand clusters). Primary piRNAs derived from these clusters are proposed to drive a ping-pong amplification cycle catalyzed by proteins that localize to the perinuclear nuage. We show that the HP1 homolog Rhino is required for nuage organization, transposon silencing, and ping-pong amplification of piRNAs. rhi mutations virtually eliminate piRNAs from the dual-strand clusters and block production of putative precursor RNAs from both strands of the major 42AB dual-strand cluster, but not of transcripts or piRNAs from the uni-strand clusters. Furthermore, Rhino protein associates with the 42AB dual-strand cluster,but does not bind to uni-strand cluster 2 or flamenco. Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle. |
| Databáze: | OpenAIRE |
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