TNF-α promoter single nucleotide polymorphisms and haplotypes associate with susceptibility of immune thrombocytopenia in Chinese adults
Autor: | Tao Liang, Gui Ning Li, Ning Wang, Lihua Hu, Xiao Bei Wang |
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Rok vydání: | 2014 |
Předmět: |
Adult
Male China Genotype Galectins Immunology Gene Expression Single-nucleotide polymorphism Immunogenetics Biology medicine.disease_cause Polymerase Chain Reaction Polymorphism Single Nucleotide Linkage Disequilibrium Autoimmunity Interferon-gamma Young Adult Sex Factors Asian People Gene Frequency immune system diseases hemic and lymphatic diseases medicine Humans Immunology and Allergy Genetic Predisposition to Disease Allele Promoter Regions Genetic DNA Primers Purpura Thrombocytopenic Idiopathic Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Haplotype General Medicine Middle Aged Exact test Haplotypes Female Tumor necrosis factor alpha Polymorphism Restriction Fragment Length |
Zdroj: | Human Immunology. 75:980-985 |
ISSN: | 0198-8859 |
Popis: | Objective Tumor necrosis factor-alpha (TNF-α) participates as a candidate susceptibility factor for immune thrombocytopenia (ITP). This study attempted to investigate the association between five single nucleotide polymorphisms (SNPs) spanning the TNF-α promoter and the susceptibility of primary ITP in Chinese Han adults. Methods In 215 adult primary ITP patients and 206 healthy controls, SNPs were detected by PCR-RFLP and PCR-SSP. The χ 2 test or fisher’s exact test was used to compare frequencies of genotypes and alleles between patients and controls. Haplotypes were analyzed with the SHEsis online program. TNF-α, IFN-γ and Galectin-9 mRNA of 35 newly diagnosed adult ITP patients and 35 healthy controls were detected by qRT-PCR. Results The haplotype GGC (−238G/−308G/−857C) of TNF-α promoter was significantly associated with a decreased susceptibility of primary ITP, especially in males. The relative levels of mRNA expression of TNF-α, IFN-γ and Gal-9 in adult active primary ITP patients was significantly up-regulated compared with patients in remission and controls. Conclusions This study represented the first report that the haplotype GGC of TNF-α was differentially associated with the susceptibility of primary ITP in Chinese Han adults. The up-regulation of TNF-α, IFN-γ and Galectin-9 was significantly correlated with active primary ITP in adult patients. |
Databáze: | OpenAIRE |
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