Non-Polar Lipids as Regulators of Membrane Properties in Archaeal Lipid Bilayer Mimics

Autor: Nicholas J. Brooks, Marta Salvador-Castell, Judith Peters, Roland Winter, Philippe Oger
Přispěvatelé: Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Microbiology of Extreme Environments (M2E), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Imperial College London, Technische Universität Dortmund [Dortmund] (TU), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Engineering & Physical Science Research Council (EPSRC), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon)
Rok vydání: 2021
Předmět:
0301 basic medicine
Hydrostatic pressure
Lipid Bilayers
02 engineering and technology
Cell membrane
chemistry.chemical_compound
Squalane
Membrane fluidity
polyisoprenoids
Biology (General)
Lipid bilayer
Spectroscopy
ComputingMilieux_MISCELLANEOUS
Liposome
Temperature
General Medicine
021001 nanoscience & nanotechnology
Computer Science Applications
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biophysics
Chemistry
Membrane
medicine.anatomical_structure
lipids (amino acids
peptides
and proteins)
fluorescence
0210 nano-technology
Laurdan
Squalene
QH301-705.5
Membrane Fluidity
0699 Other Biological Sciences
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
0399 Other Chemical Sciences
medicine
squalane
archaea
cell membrane
permeability
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
0604 Genetics
Chemical Physics
Organic Chemistry
Cell Membrane
Archaea
030104 developmental biology
chemistry
Liposomes
Biophysics
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, MDPI, 2021, 22 (11), ⟨10.3390/ijms22116087⟩
International Journal of Molecular Sciences, Vol 22, Iss 6087, p 6087 (2021)
International Journal of Molecular Sciences; Volume 22; Issue 11; Pages: 6087
'International Journal of Molecular Sciences ', vol: 22, pages: 6087-1-6087-11 (2021)
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms22116087⟩
Popis: The modification of archaeal lipid bilayer properties by the insertion of apolar molecules in the lipid bilayer midplane has been proposed to support cell membrane adaptation to extreme environmental conditions of temperature and hydrostatic pressure. In this work, we characterize the insertion effects of the apolar polyisoprenoid squalane on the permeability and fluidity of archaeal model membrane bilayers, composed of lipid analogues. We have monitored large molecule and proton permeability and Laurdan generalized polarization from lipid vesicles as a function of temperature and hydrostatic pressure. Even at low concentration, squalane (1 mol%) is able to enhance solute permeation by increasing membrane fluidity, but at the same time, to decrease proton permeability of the lipid bilayer. The squalane physicochemical impact on membrane properties are congruent with a possible role of apolar intercalants on the adaptation of Archaea to extreme conditions. In addition, such intercalant might be used to cheaply create or modify chemically resistant liposomes (archeaosomes) for drug delivery.
Databáze: OpenAIRE
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