PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus

Autor:	Eleonora Campos, Mercedes Maria Garrido, Maria Pia Valacco, Alicia S. Couto, Malena Landoni, Neil C. Bruce, Federico Sabbadin, Sonia Alejandra Wirth
Rok vydání:	2020
Předmět:	Polysaccharide complex mixtures Applied Microbiology and Biotechnology Agaricomycetes Mixed Function Oxygenases Pichia pastoris Fungal Proteins Polyporaceae 03 medical and health sciences chemistry.chemical_compound Polysaccharides Humans Cellulose Pycnoporus sanguineus 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030306 microbiology General Medicine Monooxygenase biology.organism_classification Pycnoporus Lytic cycle chemistry Biochemistry Saccharomycetales Biotechnology
Zdroj:	Applied Microbiology and Biotechnology. 104:9631-9643
ISSN:	1432-0614 0175-7598
Popis:	Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these enzymes, the recently discovered lytic polysaccharide monooxygenases (LPMOs) have revolutionized biomass processing with their novel oxidative mechanism of action. The strikingly high representation of LPMOs in fungal genomes raises the question of their functional versatility. In this work, we studied an AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus, PsAA9A. Successfully produced as a recombinant secreted protein in Pichia pastoris, PsAA9A was found to be a C1-specific LPMO active on cellulosic substrates, generating native and oxidized cello-oligosaccharides in the presence of an external electron donor. PsAA9A boosted cellulolytic activity of glysoside hydrolases from families GH1, GH5, and GH6.This study serves as a starting point towards understanding the functional versatility and biotechnological potential of this enzymatic family, highly represented in wood decay fungi, in Pycnoporus genus. KEY POINTS: • PsAA9A is the first AA9 from P. sanguineus to be characterized. • PsAA9A has activity on cellulose, producing C1-oxidized cello-oligosaccharides. • Boosting activity with GH1, GH5, and GH6 was proven.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b18f0bcd1530afd78971701135ed04c7 https://doi.org/10.1007/s00253-020-10911-6 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.