Characterization of encapsulated porcine cardiosphere-derived cells embedded in 3D alginate matrices
| Autor: | José Luis Pedraz, Kaoutar Ziani, Albert Espona-Noguera, Laura Saenz-del-Burgo, Javier G. Casado, Verónica Crisóstomo, Francisco M. Sánchez-Margallo, Jesús Ciriza |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biodistribution
Alginates Swine Alginate matrix medicine.medical_treatment Pharmaceutical Science 02 engineering and technology Enginyeria dels materials [Àrees temàtiques de la UPC] 030226 pharmacology & pharmacy Cardiac regeneration 03 medical and health sciences 0302 clinical medicine cardiosphere derived cells medicine Animals alginate Myocytes Cardiac Tissue Distribution Myocardial infarction Colloids Col·loides mesenchymal stem cells Chemistry Myocardium Regeneration (biology) Growth factor Mesenchymal stem cell Cell Differentiation Heart Myocardial infraction 021001 nanoscience & nanotechnology medicine.disease myocardial infraction 3. Good health Cell biology Infart de miocardi cell microencapsulation Heart failure Enginyeria biomèdica 0210 nano-technology Biomedical engineering Stem Cell Transplantation Ciències de la salut [Àrees temàtiques de la UPC] |
| Zdroj: | Addi. Archivo Digital para la Docencia y la Investigación instname Addi: Archivo Digital para la Docencia y la Investigación Universidad del País Vasco International Journal of Pharmaceutics UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) |
| Popis: | Myocardial infarction is caused by an interruption of coronary blood flow, leading to one of the main death causes worldwide. Current therapeutic approaches are palliative and not able to solve the loss of cardiac tissue. Cardiosphere derived cells (CDCs) reduce scarring, and increase viable myocardium, with safety and adequate biodistribution, but show a low rate engraftment and survival after implantation. In order to solve the low retention, we propose the encapsulation of CDCs within three-dimensional alginate-poly-L-lysine-alginate matrix as therapy for cardiac regeneration. In this work, we demonstrate the encapsulation of CDCs in alginate matrix, with no decrease in viability over a month, and showing the preservation of CDCs phenotype, differentiation potential, gene expression profile and growth factor release after encapsulation, moving a step forward to clinical translation of CDCs therapy in regeneration in heart failure. This work has been supported by the European Union’s H2020 Framework Program (H2020/2014-2020) and National Authorities through the Electronic Components and Systems for European Leadership Joint Undertaking (ECSEL JU) program under grant agreement Ecsel- 78132-Position-II-2017-IA. Authors also thank the support to research on cell microencapsulation from the University of the Basque Country UPV/EHU and the Basque Country Government (Grupos Consolidados, No ref: IT907-16 to J.L. P) and on CDC therapies from ISCIII (Acción Estratégica en Salud del ISCIII; Ref PI16/01172). JGC received fundings from the National Institute of Health Carlos III through a “Miguel Servet I” grant (MS17/00021) co-funded by ERDF/ESF “A way to make Europe”/“Investing in your future”, as well as fundings for the projects “CP17/00021” and “PI18/0911” (co-funded by ERDF/ESF). Authors also wish to thank the intellectual and technical assistance and from the ICTS “NANBIOSIS”, specifically by the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the University of Basque Country (UPV/EHU) in Vitoria-Gasteiz and Cell Therapy Unit (U14) at the Jesús Usón Minimally Invasive Surgery Centre. |
| Databáze: | OpenAIRE |
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