Enzyme-Mediated Lignocellulose Liquefaction Is Highly Substrate-Specific and Influenced by the Substrate Concentration or Rheological Regime
| Autor: | John N. Saddler, Timo van der Zwan, Alexander Sigg, Jinguang Hu, Richard P. Chandra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology lcsh:Biotechnology Biomedical Engineering Bioengineering 02 engineering and technology Cellulase 03 medical and health sciences Hydrolysis lignocellulose lcsh:TP248.13-248.65 Enzymatic hydrolysis Trichoderma reesei Original Research Rheometry biology Chemistry Liquefaction Substrate (chemistry) Bioengineering and Biotechnology enzymatic hydrolysis high solids enzymatic liquefaction 021001 nanoscience & nanotechnology biology.organism_classification yield stress 030104 developmental biology Chemical engineering viscosity Xylanase biology.protein rheology 0210 nano-technology Biotechnology |
| Zdroj: | Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) |
| ISSN: | 2296-4185 |
| Popis: | The high viscosities/yield stresses of lignocellulose slurries makes their industrial processing a significant challenge. However, little is known regarding the degree to which liquefaction and its enzymatic requirements are specific to a substrate’s physicochemical and rheological properties. In the work reported here, the substrate- and rheological regime-specificities of liquefaction of various substrates were assessed using real-time in-rheometer viscometry and offline oscillatory rheometry when hydrolyzed by combinations of cellobiohydrolase (Trichoderma reesei Cel7A), endoglucanase (Humicola insolens Cel45A), glycoside hydrolase (GH) family 10 xylanase, and GH family 11 xylanase. In contrast to previous work that has suggested that endoglucanase activity dominates enzymatic liquefaction, all of the enzymes were shown to have at least some liquefaction capacity depending on the substrate and reaction conditions. The contribution of individual enzymes was found to be influenced by the rheological regime; in the concentrated regime, the cellobiohydrolase outperformed the endoglucanase, achieving 2.4-fold higher yield stress reduction over the same timeframe, whereas the endoglucanase performed best in the semi-dilute regime. It was apparent that the significant differences in rheology and liquefaction mechanisms made it difficult to predict the liquefaction capacity of an enzyme or enzyme cocktail at different substrate concentrations. |
| Databáze: | OpenAIRE |
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