Epithelial SCAP/INSIG/SREBP signaling regulates multiple biological processes during perinatal lung maturation
| Autor: | Yan Xu, Angelica Schehr, Liya Huo, Machiko Ikegami, James P. Bridges, Jeffrey A. Whitsett, Yanhua Wang, Valérie Besnard |
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| Rok vydání: | 2013 |
| Předmět: |
Pulmonology
Physiology Respiratory System lcsh:Medicine Mice Cell Signaling Molecular Cell Biology Transcriptional regulation Medicine and Health Sciences lcsh:Science Sterol Regulatory Element Binding Proteins Multidisciplinary Wnt signaling pathway Intracellular Signaling Peptides and Proteins Genomics Signaling Cascades Cell biology Functional Genomics Lipogenesis lipids (amino acids peptides and proteins) Signal transduction Anatomy Transcriptome Analysis Network Analysis Signal Transduction Research Article medicine.medical_specialty Computer and Information Sciences Transgene Mice Transgenic Biology Cell Line Respiratory Failure Internal medicine medicine Genetics Animals Respiratory Physiology Regulatory Networks lcsh:R Membrane Proteins Biology and Life Sciences Computational Biology Epithelial Cells Phospholipid transport Cell Biology Genome Analysis Sterol regulatory element-binding protein Pulmonary Alveoli Endocrinology Phospholipid Signaling Cascade lcsh:Q Genome Expression Analysis Homeostasis |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 5, p e91376 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Pulmonary surfactant is required for lung function at birth and throughout postnatal life. Defects in the surfactant system are associated with common pulmonary disorders including neonatal respiratory distress syndrome and acute respiratory distress syndrome in children and adults. Lipogenesis is essential for the synthesis of pulmonary surfactant by type II epithelial cells lining the alveoli. This study sought to identify the role of pulmonary epithelial SREBP, a transcriptional regulator of cellular lipid homeostasis, during a critical time period of perinatal lung maturation in the mouse. Genome wide mRNA expression profiling of lung tissue from transgenic mice with epithelial-specific deletions of Scap (Scap(Δ/Δ), resulting in inactivation of SREBP signaling) or Insig1 and Insig2 (Insig1/2(Δ/Δ), resulting in activation of SREBP signaling) was assessed. Differentially expressed genes responding to SREBP perturbations were identified and subjected to functional enrichment analysis, pathway mapping and literature mining to predict upstream regulators and transcriptional networks regulating surfactant lipid homeostasis. Through comprehensive data analysis and integration, time dependent effects of epithelial SCAP/INSIG/SREBP deletion and defined SCAP/INSIG/SREBP-associated genes, bioprocesses and downstream pathways were identified. SREBP signaling influences epithelial development, cell death and cell proliferation at E17.5, while primarily influencing surfactant physiology, lipid/sterol synthesis, and phospholipid transport after birth. SREBP signaling integrated with the Wnt/β-catenin and glucocorticoid receptor signaling pathways during perinatal lung maturation. SREBP regulates perinatal lung lipogenesis and maturation through multiple mechanisms by interactions with distinct sets of regulatory partners. |
| Databáze: | OpenAIRE |
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