Cloning and characterization of a gene encoding a cold-shock protein in Lactobacillus casei
| Autor: | Axel Hartke, Nicolas Sauvageot, Sophie Beaufils, Alain Mazé, Josef Deutscher |
|---|---|
| Přispěvatelé: | Laboratoire de Microbiologie de l’Environnement (LME), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Microbiologie de l’Environnement ( LME ), Institut National de la Recherche Agronomique ( INRA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Microbiologie et Génétique Moléculaire ( MGM ), Institut National de la Recherche Agronomique ( INRA ) -Institut National Agronomique Paris-Grignon ( INA P-G ) -Centre National de la Recherche Scientifique ( CNRS ), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Sequence Analysis
DNA MESH : Molecular Sequence Data MESH: Amino Acid Sequence MESH: Base Sequence Start codon Lactobacillus Freezing MESH : Bacterial Proteins Cloning Molecular Peptide sequence MESH: Bacterial Proteins 0303 health sciences MESH: Gene Expression Regulation Bacterial MESH: Lactobacillus casei Inverse polymerase chain reaction MESH : Amino Acid Sequence Bacterial MESH : Cold food and beverages MESH : Heat-Shock Response Cold Temperature Lacticaseibacillus casei Biochemistry MESH : Mutation Sequence Analysis Lactobacillus casei MESH : Gene Expression Regulation Bacterial MESH : Lactobacillus casei MESH: Mutation MESH: Cold MESH : Cloning Molecular Molecular Sequence Data [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Biology Microbiology 03 medical and health sciences Bacterial Proteins Genetics MESH: Cloning Molecular Amino Acid Sequence Molecular Biology Gene 030304 developmental biology MESH: Molecular Sequence Data Base Sequence 030306 microbiology Wild type Molecular [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Gene Expression Regulation Bacterial Sequence Analysis DNA DNA Cold-shock domain biology.organism_classification Molecular biology MESH : Freezing Gene Expression Regulation MESH: Heat-Shock Response Mutation MESH : Base Sequence Heat-Shock Response Cold Cloning MESH : Sequence Analysis DNA MESH: Freezing |
| Zdroj: | FEMS Microbiology Letters FEMS Microbiology Letters, Wiley-Blackwell, 2006, 254 (1), pp.55-62. ⟨10.1111/j.1574-6968.2005.00006.x⟩ FEMS Microbiology Letters / FEMS Microbiological Letters FEMS Microbiology Letters / FEMS Microbiological Letters, 2006, 254 (1), pp.55-62. 〈10.1111/j.1574-6968.2005.00006.x〉 |
| ISSN: | 0378-1097 1574-6968 |
| DOI: | 10.1111/j.1574-6968.2005.00006.x⟩ |
| Popis: | One csp-like gene, called cspA, from the lactic acid bacterium Lactobacillus casei was identified by an inverse polymerase chain reaction approach based on degenerate primers. cspA encodes a protein of 66 amino acid residues, which displays at least 74% identity with Csp proteins of Lactobacillus genera. Northern blot analysis revealed that cspA is transcribed monocistronically and that its expression is induced after a temperature downshift from 37 degrees C to 20 degrees C. The transcriptional start site has been determined and is situated 98 bp upstream of the initiation codon. A cspA mutant strain was constructed and it showed reduced growth rate compared with the wild type at both optimal and low temperatures, demonstrating that CspA plays an important role in the physiology of L. casei. |
| Databáze: | OpenAIRE |
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