In vitro biological activity of Salvia fruticosa Mill. infusion against amyloid β-peptide-induced toxicity and inhibition of GSK-3β, CK-1δ, and BACE-1 enzymes relevant to Alzheimer's disease
| Autor: | Miyase Gözde Gündüz, L. Ömür Demirezer, Concepción Pérez, Alim Hüseyin Dokumaci, Mükerrem Betül Yerer, Mehmet Yavuz Paksoy, Perihan Gürbüz, Fatih Göger |
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| Přispěvatelé: | Erciyes University |
| Rok vydání: | 2021 |
| Předmět: |
Amyloid beta
Pharmaceutical Science Molecular modeling Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Salvia fruticosa GSK-3 medicine Neurotoxicity IC50 030304 developmental biology 0303 health sciences Lamiaceae biology Chemistry Rosmarinic acid lcsh:RM1-950 Biological activity biology.organism_classification medicine.disease Neuroprotection lcsh:Therapeutics. Pharmacology biology.protein Original Article Casein kinase 1 030217 neurology & neurosurgery geographic locations |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Saudi Pharmaceutical Journal, Vol 29, Iss 3, Pp 236-243 (2021) Saudi Pharmaceutical Journal : SPJ |
| Popis: | The authors would like to thank Dr. Ana Martinez (CSIC-CIB) for her kind and valuable support concerning the enzyme assays. Also many thanks to Dr. Ahmet Cumaog ̆lu for processing and arranging the Western blotting figures for the manuscript. MGG would like to thank Prof. Dr. Gerhard Wolber for providing the license for LigandScout 4.2 Salvia species have been traditionally used to improve cognition and have been proved to be a potential natural treatment for Alzheimer's disease. Salvia fruticosa Mill. (Turkish sage or Greek sage) demonstrated to have anticholinergic effects in vitro. The aim of this study was to understand the mechanism underlying the neuroprotective effects of S. fruticosa infusion and its representative compound rosmarinic acid, which was detected by LC-DAD-ESI-MS/MS. The protective effects of the S. fruticosa infusion (SFINF) and its major substance rosmarinic acid (RA) on amyloid beta 1–42 -induced cytotoxicity on SH-SY5Y cells together with p-GSK-3β activation were investigated. Their in vitro inhibitory effects against glycogen synthase kinase 3β, β-secretase, and casein kinase 1δ enzymes were also evaluated. The results showed that treatment with the all tested concentrations, SFINF significantly decreased Aβ 1–42-induced cytotoxicity and exhibited promising in vitro glycogen synthase kinase 3β inhibitory activity below 10 µg/mL (IC 6.52 ± 1.14 µg/mL), in addition to β-secretase inhibition (IC 86 ± 2.9 µg/mL) and casein kinase 1δ inhibition (IC 121.57 ± 4.00). The SFINF (100 µg/mL and 250 µg/mL) also activated the expression of p-GSK-3β in amyloid beta 1–42 treated SH-SY5Y cells. The outcomes of this study demonstrated that the S. fruticosa infusion possessed activity to prevent amyloid beta 1–42 -induced neurotoxicity and provided proof that its mechanism may involve regulation of p-GSK-3β protein. The authors are indebted to the Research Foundation of Erciyes University (TCD-2012-4006, FCD-2018-7834) for financially sup- porting the project. |
| Databáze: | OpenAIRE |
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