Reduction of DNA Folding by Ionic Liquids and Its Effects on the Analysis of DNA-Protein Interaction Using Solid-State Nanopore
| Autor: | Ke Luo, Young-Rok Kim, Jae-Seok Yu, Min-Cheol Lim, Jong-Yoon Jung, Ki-Baek Jeong, Ki-Bum Kim |
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| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Chemistry Biomolecule DNA Folding 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Biomaterials Hydrophobic effect Folding (chemistry) Nanopore chemistry.chemical_compound Biophysics General Materials Science Protein–DNA interaction Binding site 0210 nano-technology DNA Biotechnology |
| Zdroj: | Small. 14:1801375 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201801375 |
| Popis: | DNA folding is not desirable for solid-state nanopore techniques when analyzing the interaction of a biomolecule with its specific binding sites on DNA since the signal derived from the binding site could be buried by a large signal from the folding of DNA nearby. To resolve the problems associated with DNA folding, ionic liquids (ILs), which are known to interact with DNA through charge-charge and hydrophobic interactions are employed. 1-n-butyl-3-methylimidazolium chloride (C4 mim) is found to be the most effective in lowering the incident of DNA folding during its translocation through solid-state nanopores (4-5 nm diameter). The rate of folding signals from the translocation of DNA-C4 mim is decreased by half in comparison to that from the control bare DNA. The conformational changes of DNA upon complexation with C4 mim are further examined using atomic force microscopy, showing that the entanglement of DNA which is common in bare DNA is not observed when treated with C4 mim. The stretching effect of C4 mim on DNA strands improves the detection accuracy of nanopore for identifying the location of zinc finger protein bound to its specific binding site in DNA by lowering the incident of DNA folding. |
| Databáze: | OpenAIRE |
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