LACCASE5 Is Required for Lignification of the Brachypodium distachyon Culm
| Autor: | Oumaya Bouchabke-Coussa, Camille Soulhat, Grégory Mouille, Catherine Lapierre, Halima Morin, Frédéric Legée, Sébastien Antelme, Yin Wang, Laurent Cézard, Marion Dalmais, Philippe Lebris, Emilie Gineau, Abdelafid Bendahmane, Richard Sibout |
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| Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, unité mixte CNRS, Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique program Amelioration de la Ligno-Cellulose, European Commission’s Seventh Framework Programme project RENEWALL (grant no. 211982), the Knowledge-Based Bio Economy project CELLWALL, and BRAVO (grant no. ANR–14–CE19–0012–01), China Scholarship Council, ANR-14-CE19-0012,BRAVO,Ontogénie des tissus vasculaires chez les graminées(2014), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Université d'Évry-Val-d'Essonne (UEVE), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
2. Zero hunger
Laccase Messenger RNA biology Physiology [SDV]Life Sciences [q-bio] Mutant food and beverages Plant Science biology.organism_classification Ferulic acid chemistry.chemical_compound Hydrolysis Biochemistry chemistry Botany [SDV.IDA]Life Sciences [q-bio]/Food engineering Genetics biology.protein Lignin [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Brachypodium distachyon Peroxidase |
| Zdroj: | Plant Physiology Plant Physiology, 2015, 168 (1), pp.192--U951. ⟨10.1104/pp.114.255489⟩ Plant Physiology, American Society of Plant Biologists, 2015, 168 (1), pp.192-204. ⟨10.1104/pp.114.255489⟩ Plant Physiology, American Society of Plant Biologists, 2015, 168 (1), pp.192--U951. ⟨10.1104/pp.114.255489⟩ Plant Physiology, American Society of Plant Biologists, 2015, 168, pp.192--U951 |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.114.255489⟩ |
| Popis: | The oxidation of monolignols is a required step for lignin polymerization and deposition in cell walls. In dicots, both peroxidases and laccases are known to participate in this process. Here, we provide evidence that laccases are also involved in the lignification of Brachypodium distachyon, a model plant for temperate grasses. Transcript quantification data as well as in situ and immunolocalization experiments demonstrated that at least two laccases (LACCASE5 and LACCASE6) are present in lignifying tissues. A mutant with a misspliced LACCASE5 messenger RNA was identified in a targeting-induced local lesion in genome mutant collection. This mutant shows 10% decreased Klason lignin content and modification of the syringyl-to-guaiacyl units ratio. The amount of ferulic acid units ester linked to the mutant cell walls is increased by 40% when compared with control plants, while the amount of ferulic acid units ether linked to lignins is decreased. In addition, the mutant shows a higher saccharification efficiency. These results provide clear evidence that laccases are required for B. distachyon lignification and are promising targets to alleviate the recalcitrance of grass lignocelluloses. |
| Databáze: | OpenAIRE |
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