Corrigendum: Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit
| Autor: | Sue Biggins, Bungo Akiyoshi, Christian R. Nelson, Jeffrey A. Ranish |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Proteomics
Saccharomyces cerevisiae Proteins Kinetochore Protein subunit Cell Cycle Computational biology macromolecular substances Saccharomyces cerevisiae Biology environment and public health Yeast enzymes and coenzymes (carbohydrates) Cytoskeletal Proteins 14-3-3 Proteins Gene Expression Regulation Fungal Protein Phosphatase 1 Genetics biological phenomena cell phenomena and immunity Chromosomes Fungal Corrigendum Kinetochores Developmental Biology Research Paper Protein Binding |
| Popis: | The kinetochore is a macromolecular complex that controls chromosome segregation and cell cycle progression. When sister kinetochores make bioriented attachments to microtubules from opposite poles, the spindle checkpoint is silenced. Biorientation and the spindle checkpoint are regulated by a balance between the Ipl1/Aurora B protein kinase and the opposing activity of protein phosphatase I (PP1). However, little is known about the regulation of PP1 localization and activity at the kinetochore. Here, we developed a method to purify centromere-bound kinetochores and used quantitative proteomics to identify the Fin1 protein as a PP1 regulatory subunit. The Fin1/PP1 complex is regulated by phosphorylation and 14–3–3 protein binding. When Fin1 is mislocalized, bipolar spindles fail to assemble but the spindle checkpoint is inappropriately silenced due to PP1 activity. These data suggest that Fin1 is a PP1 regulatory subunit whose spatial and temporal activity must be precisely controlled to ensure genomic stability. |
| Databáze: | OpenAIRE |
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