Interleukin-1 β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study
| Autor: | Dorota Tomaszewska-Zaremba, Janina Skipor, Anna Herman, Karolina Król, Hanna Antushevich, Joanna Bochenek, Agata Krawczyńska, E. Marciniak, Bartosz Pawlina |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Acetylserotonin O-Methyltransferase
Male medicine.medical_specialty endocrine system Lipopolysaccharide Article Subject Interleukin-1beta lcsh:Medicine Biology Arylalkylamine N-Acetyltransferase Pineal Gland General Biochemistry Genetics and Molecular Biology Gene Expression Regulation Enzymologic Proinflammatory cytokine Melatonin chemistry.chemical_compound Pineal gland Norepinephrine Internal medicine Gene expression medicine Animals RNA Messenger Sheep General Immunology and Microbiology lcsh:R Interleukin General Medicine medicine.anatomical_structure Endocrinology chemistry Acetylserotonin O-methyltransferase Female Ex vivo medicine.drug Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2015/526464 |
| Popis: | The study was designed to determine the effect of proinflammatory cytokine, interleukin- (IL-) 1β, on melatonin release and expression enzymes essential for this hormone synthesis: arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in ovine pineal gland, taking into account the immune status of animals before sacrificing. Ewes were injected by lipopolysaccharide (LPS; 400 ng/kg) or saline, two hours after sunset during short day period (December). Animals were euthanized three hours after the injection. Next, the pineal glands were collected and divided into four explants. The explants were incubated with (1) medium 199 (control explants), (2) norepinephrine (NE; 10 µM), (3) IL-1β(75 pg/mL), or (4) NE + IL-1β. It was found that IL-1βabolished (P<0.05) NE-induced increase in melatonin release. Treatment with IL-1βalso reduced (P<0.05) expression of AA-NAT enzyme compared to NE-treated explants. There was no effect of NE or IL-1βtreatment on gene expression of HIOMT; however, the pineal fragments isolated from LPS-treated animals were characterized by elevated (P<0.05) expression of HIOMT mRNA and protein compared to the explants from saline-treated ewes. Our study proves that IL-1βsuppresses melatonin secretion and its action seems to be targeted on the reduction of pineal AA-NAT protein expression. |
| Databáze: | OpenAIRE |
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