Identification and characterization of an R-Smad homologue (Hco-DAF-8) from Haemonchus contortus
| Autor: | Feng Liu, Chunqun Wang, Caixian Zhou, Fangfang Li, Jia-Nan Shan, Li He, Wenda Di, Rui Fang, Min Hu, Robin B. Gasser |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Gene rescue SMAD Development R-Smads lcsh:Infectious and parasitic diseases Transcriptome Transcription (biology) Haemonchus contortus TGF beta signaling pathway parasitic diseases Animals lcsh:RC109-216 Caenorhabditis elegans Caenorhabditis elegans Proteins Gene Specific inhibitor of human Smad3 (SIS3) R-SMAD biology Research Gene Expression Profiling fungi TGF-β signalling pathway Computational Biology Genomics Helminth Proteins biology.organism_classification Smad Proteins Receptor-Regulated Cell biology Infectious Diseases Parasitology Female Haemonchus Sequence Alignment Signal Transduction |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 13, Iss 1, Pp 1-13 (2020) |
| ISSN: | 1756-3305 |
| Popis: | BackgroundSmad proteins are essential cellular mediators within the transforming growth factor-β (TGF-β) superfamily. They directly transmit incoming signals from the cell surface receptors to the nucleus. In spite of their functional importance, almost nothing is known about Smad proteins in parasitic nematodes includingHaemonchus contortus, an important blood-sucking nematode of small ruminants.MethodsBased on genomic and transcriptome data forH. contortusand using bioinformatics methods, a Smad homologue (calledHco-daf-8) was inferred fromH.contortusand the structural characteristics of this gene and its encoded proteinHco-DAF-8 established. Using real-time PCR and immunofluorescence assays, temporal transcriptional and spatial expression profiles ofHco-daf-8were studied. Gene rescue inCaenorhabditis eleganswas then applied to assess the function ofHco-daf-8and a specific inhibitor of human Smad3 (called SIS3) was employed to evaluate the roles ofHco-DAF-8 inH. contortusdevelopment.ResultsThe features ofHco-DAF-8 (502 amino acids), including conserved R-Smad domains and residues of the L3-loop that determine pathway specificity, are consistent with a TGF-β type I receptor-activated R-Smad. TheHco-daf-8gene was transcribed in all developmental stages ofH. contortusstudied, with a higher level of transcription in the fourth-stage larval (L4) females and the highest level in adult males.Hco-DAF-8 was expressed in the platymyarian muscular cells, intestine and reproductive system of adult stages. Gene rescue experiments showed thatHco-daf-8was able to partially rescue gene function in adaf-8deficient mutant strain ofC. elegans, leading to a resumption of normal development. InH. contortus, SIS3 was shown to affectH. contortusdevelopment from the exsheathed third-stage larvae (L3s) to L4sin vitro.ConclusionsThese findings suggest thatHco-DAF-8, encoded by the geneHco-daf-8, is an important cellular mediator ofH. contortusdevelopmentviathe TGF-β signalling pathway. They provide a basis for future explorations ofHco-DAF-8 and associated pathways inH. contortusand other important parasitic nematodes. |
| Databáze: | OpenAIRE |
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