Mechanisms underlying the cytotoxic effect of propolis on human laryngeal epidermoid carcinoma cells
| Autor: | Alexis Díaz-García, Hermis Rodríguez-Sánchez, José Maurício Sforcin, Yahima Frión-Herrera, Jenny Laura Ruiz-Fuentes |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Laboratories of Biofarmaceuticals and Chemistries Productions (LABIOFAM), Tropical Medicine Institute Pedro Kouri |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Apoptosis Plant Science Pharmacology 01 natural sciences Biochemistry HEp-2 cells Propolis Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation Cell Line Tumor Lactate dehydrogenase Carcinoma medicine Humans Cytotoxic T cell Viability assay Cytotoxicity Laryngeal Neoplasms Membrane Potential Mitochondrial Chemistry Organic Chemistry apoptosis medicine.disease 0104 chemical sciences 010404 medicinal & biomolecular chemistry 030104 developmental biology cytotoxicity Reactive Oxygen Species Brazil |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2017.1363749 |
| Popis: | Made available in DSpace on 2018-12-11T17:13:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-09-02 Propolis has been used as a traditional remedy for centuries because of its beneficial effects, including anticancer properties. The aim of this study was to compare the cytotoxic mechanism of Cuban red propolis (CP) and Brazilian green propolis (BP) on human laryngeal carcinoma (HEp-2) cells. Cell viability, leakage of lactate dehydrogenase, fluorescence staining, mitochondrial membrane potential (ΔΨm) and the expression of pro/anti-apoptotic genes were assessed. Cell viability and cytotoxic assays suggested a dose-dependent effect of CP and BP extracts with a possible association of intracellular reactive oxygen species production and decreased ΔΨm. Both samples induced apoptosis via activation of TP53, CASP3, BAX, P21 signalling, and downregulation of BCL2 and BCL-XL. CP exerted a higher cytotoxic effect than BP extract. Our findings suggest further investigation of the main components of each propolis sample, what may lead to the development of strategies for the treatment of laryngeal cancer. Department of Microbiology and Immunology Biosciences Institute UNESP Laboratories of Biofarmaceuticals and Chemistries Productions (LABIOFAM) Tropical Medicine Institute Pedro Kouri Department of Microbiology and Immunology Biosciences Institute UNESP |
| Databáze: | OpenAIRE |
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