Dual role of the Cdc7-regulatory protein Dbf4 during yeast meiosis
| Autor: | Etienne Schwob, Flavio Della Seta, Guillaume Valentin |
|---|---|
| Přispěvatelé: | Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins Cell Cycle Proteins Protein Serine-Threonine Kinases Biology Biochemistry Genetic recombination Chromosomes S Phase Chromosome segregation 03 medical and health sciences 0302 clinical medicine Meiosis Chromosome Segregation Yeasts Homologous chromosome [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Interkinesis Molecular Biology Anaphase Cell Cycle Proteins/*physiology Chromosome Segregation Chromosomes DNA Replication Meiosis/*genetics Protein Kinases S Phase Saccharomyces cerevisiae Proteins/*physiology Yeasts/*cytology/physiology 030304 developmental biology Anaphase Genetics 0303 health sciences Cohesin Cell Biology Meiotic chromosome segregation Protein Kinases 030217 neurology & neurosurgery |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281 (5), pp.2828--34. ⟨10.1074/jbc.M510626200⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M510626200⟩ |
| Popis: | International audience; The Dbf4-dependent Cdc7 kinase (DDK) is essential for chromosome duplication in all eukaryotes, but was proposed to be dispensable for yeast pre-meiotic DNA replication. This discrepancy led us to investigate the role of the unstable Cdc7-regulatory protein Dbf4 in meiosis. We show that, when Dbf4 is depleted at the time of meiotic induction, cells enter the meiotic program but do not replicate their chromosomes. Surprisingly when Dbf4 is depleted after the initiation of DNA synthesis, S phase goes to completion, but most cells arrest before anaphase I. Deletion of the cohesin Rec8 suppresses this phenotype, suggesting a distinct role of DDK for meiotic chromosome segregation. As after Cdc5 depletion, a fraction of cells undergo a single equational division suggesting a failure to mono-orient sister kinetochores. Our results demonstrate that Dbf4 is essential for DNA replication during meiosis like in vegetative cells and provide evidence for an additional role in setting up the reductional division of meiosis I. |
| Databáze: | OpenAIRE |
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