Proteomic study of hypothalamus in pigs exposed to heat stress
| Autor: | Xiaoxi Liu, Ravi Gooneratne, Yanhong Yong, Canying Hu, Tianyue Yu, Zhichao Yu, Lianyun Wu, Yadnyavalkya Patil, Biao Fang, Xianghong Ju, Xingbin Ma, Junyu Li |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics medicine.medical_specialty Pituitary gland Swine 040301 veterinary sciences Quantitative proteomics Hypothalamus Biology Weight Gain Heat stress Body Temperature 0403 veterinary science 03 medical and health sciences Immune system Internal medicine medicine Animals Secretion 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary 04 agricultural and veterinary sciences General Medicine medicine.anatomical_structure Endocrinology Proteome lcsh:SF600-1100 Swine Miniature Pigs Heat-Shock Response Function (biology) Research Article Hormone |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 16, Iss 1, Pp 1-11 (2020) |
| ISSN: | 1746-6148 |
| Popis: | BackgroundWith evidence of warming climates, it is important to understand the effects of heat stress in farm animals in order to minimize production losses. Studying the changes in the brain proteome induced by heat stress may aid in understanding how heat stress affects brain function. The hypothalamus is a critical region in the brain that controls the pituitary gland, which is responsible for the secretion of several important hormones. In this study, we examined the hypothalamic protein profile of 10 pigs (15 ± 1 kg body weight), with five subjected to heat stress (35 ± 1 °C; relative humidity = 90%) and five acting as controls (28 ± 3 °C; RH = 90%).ResultThe isobaric tags for relative and absolute quantification (iTRAQ) analysis of the hypothalamus identified 1710 peptides corresponding to 360 proteins, including 295 differentially expressed proteins (DEPs), 148 of which were up-regulated and 147 down-regulated, in heat-stressed animals. The Ingenuity Pathway Analysis (IPA) software predicted 30 canonical pathways, four functional groups, and four regulatory networks of interest. The DEPs were mainly concentrated in the cytoskeleton of the pig hypothalamus during heat stress.ConclusionsIn this study, heat stress significantly increased the body temperature and reduced daily gain of body weight in pigs. Furthermore, we identified 295 differentially expressed proteins, 147 of which were down-regulated and 148 up-regulated in hypothalamus of heat stressed pigs. The IPA showed that the DEPs identified in the study are involved in cell death and survival, cellular assembly and organization, and cellular function and maintenance, in relation to neurological disease, metabolic disease, immunological disease, inflammatory disease, and inflammatory response. We hypothesize that a malfunction of the hypothalamus may destroy the host physical and immune function, resulting in decreased growth performance and immunosuppression in heat stressed pigs. |
| Databáze: | OpenAIRE |
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