Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading

Autor:	Gideon Coster, John F.X. Diffley
Rok vydání:	2017
Předmět:	DNA Replication 0301 basic medicine Saccharomyces cerevisiae Proteins Origin Recognition Complex Replication Origin Eukaryotic DNA replication Saccharomyces cerevisiae Biology Article 03 medical and health sciences chemistry.chemical_compound Minichromosome maintenance Control of chromosome duplication Binding site Genetics Binding Sites Multidisciplinary Minichromosome Maintenance Proteins DNA Helicases DNA replication Helicase Cell biology 030104 developmental biology chemistry biology.protein Origin recognition complex DNA
Zdroj:	Science
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.aan0063
Popis:	Getting loaded—make mine a double! Chromosomal DNA replication initiates bidirectionally by loading two ring-shaped helicases onto DNA in opposite orientations. How this symmetry is achieved has been puzzling because replication initiation sites contain only one essential binding site for the initiator, the origin recognition complex (ORC). Coster and Diffley now show that both helicases are loaded by a similar mechanism. Efficient loading requires binding of two ORC complexes to two ORC binding sites in opposite orientations. Natural origins were found to be partially symmetrical, containing functionally relevant secondary ORC sites. Sites can be flexibly spaced, but introducing an intervening “roadblock” prevented loading, suggesting that individual helicases translocate toward each other on DNA to form a stable double ring. Science , this issue p. 314
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b181a46e89a8d0a7074a9781f8ff3644 https://doi.org/10.1126/science.aan0063 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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