Mass spectrometry characterisation of fatty acids from metabolically engineered soybean seeds
| Autor: | Alex M. Machado, Valquiria A. M. Lacerda, Elíbio L. Rech, Giovanni R. Vianna, Nicolau B. da Cunha, Cintia M. Coelho, Marly Coelho, André M. Murad |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Biodiesel food.ingredient Fatty Acids food and beverages Fatty acid Plants Genetically Modified Biochemistry Soybean oil Soybean Oil Analytical Chemistry Freezing point Palmitic acid Metabolic engineering chemistry.chemical_compound Oleic acid food Metabolic Engineering chemistry Seeds Soybeans Gas chromatography Food science |
| Zdroj: | Analytical and Bioanalytical Chemistry. 406:2873-2883 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | Improving the quality and performance of soybean oil as biodiesel depends on the chemical composition of its fatty acids and requires an increase in monounsaturated acids and a reduction in polyunsaturated acids. Despite its current use as a source of biofuel, soybean oil contains an average of 25 % oleic acid and 13 % palmitic acid, which negatively impacts its oxidative stability and freezing point, causing a high rate of nitrogen oxide emission. Gas chromatography and ion mobility mass spectrometry were conducted on soybean fatty acids from metabolically engineered seed extracts to determine the nature of the structural oleic and palmitic acids. The soybean genes FAD2-1 and FatB were placed under the control of the 35SCaMV constitutive promoter, introduced to soybean embryonic axes by particle bombardment and down-regulated using RNA interference technology. Results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 94.58 %) and a reduction in palmitic acid (to |
| Databáze: | OpenAIRE |
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