Identification of co-expression modules and pathways correlated with osteosarcoma and its metastasis
| Autor: | Jing-ming Han, Yong-Sheng Zhong, Peng Xie, Xue-dong Li, Jian‑Sheng Wang, Shi-xin Du, Yun-guo Wang, Gui-zhou Zheng |
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| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
Microtubule bundle formation lcsh:Surgery Gene Expression Bone Neoplasms lcsh:RC254-282 Metastasis 03 medical and health sciences 0302 clinical medicine Surgical oncology medicine Humans Neoplasm Metastasis Pathways neoplasms Gene Osteosarcoma business.industry Research Gene Expression Profiling DNA replication lcsh:RD1-811 Pathway enrichment lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Prognosis medicine.disease Co-expression modules Gene Expression Regulation Neoplastic Oncology 030220 oncology & carcinogenesis Cancer research 030211 gastroenterology & hepatology Surgery Signal transduction business |
| Zdroj: | World Journal of Surgical Oncology World Journal of Surgical Oncology, Vol 17, Iss 1, Pp 1-7 (2019) |
| ISSN: | 1477-7819 |
| DOI: | 10.1186/s12957-019-1587-7 |
| Popis: | Background Osteosarcoma is the most common bone tumor that occurs in children. Methods To identify co-expression modules and pathways correlated with osteosarcoma and its clinical characteristics, we performed weighted gene co-expression network analysis (WGCNA) on RNA-seq data of osteosarcoma with 52 samples. Then we performed pathway enrichment analysis on genes from significant modules. Results A total of 5471 genes were included in WGCNA, and 16 modules were identified. Module-trait analysis identified that a module involved in microtubule bundle formation, drug metabolism-cytochrome P450, and IL-17 signaling pathway was negatively correlated with osteosarcoma and positively correlated with metastasis; a module involved in DNA replication was positively correlated with osteosarcoma; a module involved in cell junction was positively correlated with metastasis; and a module involved in heparin binding negatively correlated with osteosarcoma. Moreover, expression levels in four of the top ten differentially expressed genes were validated in another independent dataset. Conclusions Our analysis might provide insight for molecular mechanisms of osteosarcoma. Electronic supplementary material The online version of this article (10.1186/s12957-019-1587-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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