Distribution and Characterization of Actinomycetes in Mangrove Habitats (Red Sea, Egypt) with Special Emphasis on Streptomyces mutabilis M3MT483919
| Autor: | Hanan Abd-Elnaby, Mohamed A.A. Abdrabo, Lamis Sh. Abdelfattah, Gehan M. Abou El ela, Moaz M. Hamed, Mahmoud Saber Kelany, Nayer M. Fahmy, Sahar W.M. Hassan, Hala H. Abd El Latif |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
streptomyces mutabilis Ecology business.industry mangrove habitats 030106 microbiology Distribution (economics) m3 Biology Applied Microbiology and Biotechnology Microbiology Streptomyces mutabilis QR1-502 03 medical and health sciences 030104 developmental biology Habitat Mangrove business Biotechnology |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 15, Iss 1, Pp 246-261 (2021) |
| ISSN: | 2581-690X 0973-7510 |
| DOI: | 10.22207/jpam.15.1.19 |
| Popis: | Ten sediment samples were gathered from several geographical locations around mangrove habitat, Red Sea coast, Egypt, during summer 2019. Actinobacteria are widespread in most mangrove soil samples. The average actinomycetes counts in sediment samples were ranged from 4 to 15 CFUg-1, also physico-chemical characters for soil samples were determined. Statistical analysis was applied to assess if the geographical location and physico-chemical characters influenced the communities of actinomycetes. A total of 10 actinomycetes were isolated and characterized physiologically and biochemically. The antimicrobial activities of different actinomycetes isolates were assessed. Isolate M3 was chosen as the most promising isolate with broad antagonistic activity against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 19404, Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC 9027, and Candida albicans ATCC 10231 with inhibition zones ranged from 12.0 ± 0.9 to 20.0 ± 1.9 mm. Genotypic characterization of isolate M3 was made using 16S rDNA sequence analysis and identified as Streptomyces mutabilis M3 with accession number MT483919. This strain exhibited anticancer activity against breast cancer cell line (Mcf7), liver cancer cell line (HepG2) and colon cancer cell line (HCT116) and the IC50 values were 324.77, 333.71 and 354.46, respectively. Streptomyces mutabilis M3 MT483919 had high bio-flocculating activity for seawater treatment, and the recovery of the samples ranged between 71.97 and 76.05%. The crude extract of Streptomyces mutabilis MT483919 M3 was analyzed by Fourier transform infrared spectrum (FT-IR) and Gas chromatography-mass spectrometry (GC-MS). |
| Databáze: | OpenAIRE |
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